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@ARTICLE{ChamorroGonzlez:275793,
      author       = {R. Chamorro González and T. Conrad and M. C. Stöber and
                      R. Xu and M. Giurgiu and E. Rodriguez-Fos and K. Kasack and
                      L. Brückner and E. van Leen and K. Helmsauer and H. Dorado
                      Garcia and M. E. Stefanova and K. L. Hung and Y. Bei and K.
                      Schmelz and M. Lodrini and S. Mundlos and H. Y. Chang and H.
                      E. Deubzer$^*$ and S. Sauer and A. Eggert$^*$ and J.
                      Schulte$^*$ and R. F. Schwarz and K. Haase$^*$ and R. P.
                      Koche and A. G. Henssen$^*$},
      title        = {{P}arallel sequencing of extrachromosomal circular {DNA}s
                      and transcriptomes in single cancer cells.},
      journal      = {Nature genetics},
      volume       = {55},
      number       = {5},
      issn         = {1061-4036},
      address      = {London},
      publisher    = {Macmillan Publishers Limited, part of Springer Nature},
      reportid     = {DKFZ-2023-00881},
      pages        = {880-890},
      year         = {2023},
      note         = {2023 May;55(5):880-890},
      abstract     = {Extrachromosomal DNAs (ecDNAs) are common in cancer, but
                      many questions about their origin, structural dynamics and
                      impact on intratumor heterogeneity are still unresolved.
                      Here we describe single-cell extrachromosomal circular DNA
                      and transcriptome sequencing $(scEC\&T-seq),$ a method for
                      parallel sequencing of circular DNAs and full-length mRNA
                      from single cells. By applying $scEC\&T-seq$ to cancer
                      cells, we describe intercellular differences in ecDNA
                      content while investigating their structural heterogeneity
                      and transcriptional impact. Oncogene-containing ecDNAs were
                      clonally present in cancer cells and drove intercellular
                      oncogene expression differences. In contrast, other small
                      circular DNAs were exclusive to individual cells, indicating
                      differences in their selection and propagation.
                      Intercellular differences in ecDNA structure pointed to
                      circular recombination as a mechanism of ecDNA evolution.
                      These results demonstrate $scEC\&T-seq$ as an approach to
                      systematically characterize both small and large circular
                      DNA in cancer cells, which will facilitate the analysis of
                      these DNA elements in cancer and beyond.},
      cin          = {BE01},
      ddc          = {570},
      cid          = {I:(DE-He78)BE01-20160331},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:37142849},
      doi          = {10.1038/s41588-023-01386-y},
      url          = {https://inrepo02.dkfz.de/record/275793},
}