TY  - JOUR
AU  - Naghavian, Reza
AU  - Faigle, Wolfgang
AU  - Oldrati, Pietro
AU  - Wang, Jian
AU  - Toussaint, Nora C
AU  - Qiu, Yuhan
AU  - Medici, Gioele
AU  - Wacker, Marcel
AU  - Freudenmann, Lena
AU  - Bonté, Pierre-Emmanuel
AU  - Weller, Michael
AU  - Regli, Luca
AU  - Amigorena, Sebastian
AU  - Rammensee, Hans-Georg
AU  - Walz, Juliane
AU  - Brugger, Silvio D
AU  - Mohme, Malte
AU  - Zhao, Yingdong
AU  - Sospedra, Mireia
AU  - Neidert, Marian C
AU  - Martin, Roland
TI  - Microbial peptides activate tumour-infiltrating lymphocytes in glioblastoma.
JO  - Nature 
VL  - 617
IS  - 7962
SN  - 0028-0836
CY  - London [u.a.]
PB  - Nature Publ. Group
M1  - DKFZ-2023-00999
SP  - 807-817
PY  - 2023
N1  - 2023 May;617(7962):807-817
AB  - Microbial organisms have key roles in numerous physiological processes in the human body and have recently been shown to modify the response to immune checkpoint inhibitors1,2. Here we aim to address the role of microbial organisms and their potential role in immune reactivity against glioblastoma. We demonstrate that HLA molecules of both glioblastoma tissues and tumour cell lines present bacteria-specific peptides. This finding prompted us to examine whether tumour-infiltrating lymphocytes (TILs) recognize tumour-derived bacterial peptides. Bacterial peptides eluted from HLA class II molecules are recognized by TILs, albeit very weakly. Using an unbiased antigen discovery approach to probe the specificity of a TIL CD4+ T cell clone, we show that it recognizes a broad spectrum of peptides from pathogenic bacteria, commensal gut microbiota and also glioblastoma-related tumour antigens. These peptides were also strongly stimulatory for bulk TILs and peripheral blood memory cells, which then respond to tumour-derived target peptides. Our data hint at how bacterial pathogens and bacterial gut microbiota can be involved in specific immune recognition of tumour antigens. The unbiased identification of microbial target antigens for TILs holds promise for future personalized tumour vaccination approaches.
LB  - PUB:(DE-HGF)16
C6  - pmid:37198490
DO  - DOI:10.1038/s41586-023-06081-w
UR  - https://inrepo02.dkfz.de/record/276099
ER  -