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000276325 041__ $$aEnglish
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000276325 1001_ $$aFresnais, Margaux$$b0
000276325 245__ $$aDesorption Kinetics Evaluation for the Development of Validated Desorption Electrospray Ionization-Mass Spectrometric Assays for Drug Quantification in Tissue Sections.
000276325 260__ $$aBasel$$bMolecular Diversity Preservation International$$c2023
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000276325 520__ $$aThe development of desorption/ionization (DI) mass spectrometric (MS) assays for drug quantification in tissue sections and their validation according to regulatory guidelines would enable their universalization for applications in (clinical) pharmacology. Recently, new enhancements in desorption electrospray ionization (DESI) have highlighted the reliability of this ion source for the development of targeted quantification methods that meet requirements for method validation. However, it is necessary to consider subtle parameters leading to the success of such method developments, such as the morphology of desorption spots, the analytical time, and sample surface, to cite but a few. Here, we provide additional experimental data highlighting an additional important parameter, based on the unique advantage of DESI-MS on continuous extraction during analysis. We demonstrate that considering desorption kinetics during DESI analyses would largely help (i) reducing analytical time during profiling analyses, (ii) verifying solvent-based drug extraction using the selected sample preparation method for profiling and imaging modes, and (iii) predicting the feasibility of imaging assays using samples in a given expected concentration range of the targeted drug. These observations will likely serve as precious guidance for the development of validated DESI-profiling and imaging methods in the future.
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000276325 650_7 $$2Other$$adesorption electrospray ionization
000276325 650_7 $$2Other$$adrug quantification
000276325 650_7 $$2Other$$amass spectrometry
000276325 650_2 $$2MeSH$$aSpectrometry, Mass, Electrospray Ionization: methods
000276325 650_2 $$2MeSH$$aReproducibility of Results
000276325 650_2 $$2MeSH$$aKinetics
000276325 650_2 $$2MeSH$$aDiagnostic Imaging
000276325 7001_ $$aLiang, Siwen$$b1
000276325 7001_ $$aSeven, Deniz$$b2
000276325 7001_ $$aProdanovic, Nevena$$b3
000276325 7001_ $$0P:(DE-He78)303cfc65bbd4cdc79617186d2267d9fd$$aSundheimer, Julia$$b4$$udkfz
000276325 7001_ $$00000-0003-0672-6876$$aHaefeli, Walter E$$b5
000276325 7001_ $$00000-0002-2190-1698$$aBurhenne, Jürgen$$b6
000276325 7001_ $$00000-0003-2003-1886$$aLonguespée, Rémi$$b7
000276325 773__ $$0PERI:(DE-600)2019364-6$$a10.3390/ijms24108469$$gVol. 24, no. 10, p. 8469 -$$n10$$p8469$$tInternational journal of molecular sciences$$v24$$x1422-0067$$y2023
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