TY  - JOUR
AU  - Fresnais, Margaux
AU  - Liang, Siwen
AU  - Seven, Deniz
AU  - Prodanovic, Nevena
AU  - Sundheimer, Julia
AU  - Haefeli, Walter E
AU  - Burhenne, Jürgen
AU  - Longuespée, Rémi
TI  - Desorption Kinetics Evaluation for the Development of Validated Desorption Electrospray Ionization-Mass Spectrometric Assays for Drug Quantification in Tissue Sections.
JO  - International journal of molecular sciences
VL  - 24
IS  - 10
SN  - 1422-0067
CY  - Basel
PB  - Molecular Diversity Preservation International
M1  - DKFZ-2023-01055
SP  - 8469
PY  - 2023
AB  - The development of desorption/ionization (DI) mass spectrometric (MS) assays for drug quantification in tissue sections and their validation according to regulatory guidelines would enable their universalization for applications in (clinical) pharmacology. Recently, new enhancements in desorption electrospray ionization (DESI) have highlighted the reliability of this ion source for the development of targeted quantification methods that meet requirements for method validation. However, it is necessary to consider subtle parameters leading to the success of such method developments, such as the morphology of desorption spots, the analytical time, and sample surface, to cite but a few. Here, we provide additional experimental data highlighting an additional important parameter, based on the unique advantage of DESI-MS on continuous extraction during analysis. We demonstrate that considering desorption kinetics during DESI analyses would largely help (i) reducing analytical time during profiling analyses, (ii) verifying solvent-based drug extraction using the selected sample preparation method for profiling and imaging modes, and (iii) predicting the feasibility of imaging assays using samples in a given expected concentration range of the targeted drug. These observations will likely serve as precious guidance for the development of validated DESI-profiling and imaging methods in the future.
KW  - Spectrometry, Mass, Electrospray Ionization: methods
KW  - Reproducibility of Results
KW  - Kinetics
KW  - Diagnostic Imaging
KW  - desorption electrospray ionization (Other)
KW  - drug quantification (Other)
KW  - mass spectrometry (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:37239813
C2  - pmc:PMC10217933
DO  - DOI:10.3390/ijms24108469
UR  - https://inrepo02.dkfz.de/record/276325
ER  -