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@ARTICLE{Saini:276368,
author = {M. Saini$^*$ and L. Schmidleitner and H. D. Moreno and E.
Donato$^*$ and M. Falcone$^*$ and J. M. Bartsch and V.
Vogel$^*$ and R. Würth$^*$ and N. Pfarr and E. Espinet$^*$
and M. Lehmann and M. Königshoff and M. Reitberger$^*$ and
S. Haas$^*$ and E. Graf and T. Schwarzmayr and T.-M. Strom
and S. Spaich and M. Sütterlin and A. Schneeweiss and W.
Weichert$^*$ and G. Schotta and M. Reichert$^*$ and N. Aceto
and M. Sprick$^*$ and A. Trumpp$^*$ and C. H. Scheel and C.
Klein$^*$},
title = {{R}esistance to mesenchymal reprogramming sustains clonal
propagation in metastatic breast cancer.},
journal = {Cell reports},
volume = {42},
number = {6},
issn = {2211-1247},
address = {[New York, NY]},
publisher = {Elsevier},
reportid = {DKFZ-2023-01077},
pages = {112533},
year = {2023},
note = {DKFZ-ZMBH Alliance / #EA:A010#LA:A010# / 2023 Jun
27;42(6):112533},
abstract = {The acquisition of mesenchymal traits is considered a
hallmark of breast cancer progression. However, the
functional relevance of epithelial-to-mesenchymal transition
(EMT) remains controversial and context dependent. Here, we
isolate epithelial and mesenchymal populations from human
breast cancer metastatic biopsies and assess their
functional potential in vivo. Strikingly, progressively
decreasing epithelial cell adhesion molecule (EPCAM) levels
correlate with declining disease propagation.
Mechanistically, we find that persistent EPCAM expression
marks epithelial clones that resist EMT induction and
propagate competitively. In contrast, loss of EPCAM defines
clones arrested in a mesenchymal state, with concomitant
suppression of tumorigenicity and metastatic potential. This
dichotomy results from distinct clonal trajectories
impacting global epigenetic programs that are determined by
the interplay between human ZEB1 and its target GRHL2.
Collectively, our results indicate that susceptibility to
irreversible EMT restrains clonal propagation, whereas
resistance to mesenchymal reprogramming sustains disease
spread in multiple models of human metastatic breast cancer,
including patient-derived cells in vivo.},
keywords = {CP: Cancer (Other) / EMT (Other) / EPCAM (Other) / GRHL2
(Other) / ZEB1 (Other) / breast cancer (Other) / intratumor
heterogeneity (Other) / metastasis (Other)},
cin = {A010 / HD01 / MU01},
ddc = {610},
cid = {I:(DE-He78)A010-20160331 / I:(DE-He78)HD01-20160331 /
I:(DE-He78)MU01-20160331},
pnm = {311 - Zellbiologie und Tumorbiologie (POF4-311)},
pid = {G:(DE-HGF)POF4-311},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:37257449},
doi = {10.1016/j.celrep.2023.112533},
url = {https://inrepo02.dkfz.de/record/276368},
}