TY  - JOUR
AU  - Roohani, Siyer
AU  - Loskutov, Jürgen
AU  - Heufelder, Jens
AU  - Ehret, Felix
AU  - Wedeken, Lena
AU  - Regenbrecht, Manuela
AU  - Sauer, Rica
AU  - Zips, Daniel
AU  - Denker, Andrea
AU  - Joussen, Antonia M
AU  - Regenbrecht, Christian R A
AU  - Kaul, David
TI  - Photon and Proton irradiation in Patient-derived, Three-Dimensional Soft Tissue Sarcoma Models.
JO  - BMC cancer
VL  - 23
IS  - 1
SN  - 1471-2407
CY  - Heidelberg
PB  - Springer
M1  - DKFZ-2023-01261
SP  - 577
PY  - 2023
AB  - Despite their heterogeneity, the current standard preoperative radiotherapy regimen for localized high-grade soft tissue sarcoma (STS) follows a one fits all approach for all STS subtypes. Sarcoma patient-derived three-dimensional cell culture models represent an innovative tool to overcome challenges in clinical research enabling reproducible subtype-specific research on STS. In this pilot study, we present our methodology and preliminary results using STS patient-derived 3D cell cultures that were exposed to different doses of photon and proton radiation. Our aim was: (i) to establish a reproducible method for irradiation of STS patient-derived 3D cell cultures and (ii) to explore the differences in tumor cell viability of two different STS subtypes exposed to increasing doses of photon and proton radiation at different time points.Two patient-derived cell cultures of untreated localized high-grade STS (an undifferentiated pleomorphic sarcoma (UPS) and a pleomorphic liposarcoma (PLS)) were exposed to a single fraction of photon or proton irradiation using doses of 0 Gy (sham irradiation), 2 Gy, 4 Gy, 8 Gy and 16 Gy. Cell viability was measured and compared to sham irradiation at two different time points (four and eight days after irradiation).The proportion of viable tumor cells four days after photon irradiation for UPS vs. PLS were significantly different with 85
KW  - Organoid (Other)
KW  - Patient-derived (Other)
KW  - Photon (Other)
KW  - Proton (Other)
KW  - Radiotherapy (Other)
KW  - Sarcoma, 3D cell culture (Other)
KW  - Soft tissue sarcoma (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:37349697
DO  - DOI:10.1186/s12885-023-11013-y
UR  - https://inrepo02.dkfz.de/record/277077
ER  -