% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Roohani:277077,
      author       = {S. Roohani$^*$ and J. Loskutov and J. Heufelder and F.
                      Ehret$^*$ and L. Wedeken and M. Regenbrecht and R. Sauer and
                      D. Zips$^*$ and A. Denker and A. M. Joussen and C. R. A.
                      Regenbrecht and D. Kaul$^*$},
      title        = {{P}hoton and {P}roton irradiation in {P}atient-derived,
                      {T}hree-{D}imensional {S}oft {T}issue {S}arcoma {M}odels.},
      journal      = {BMC cancer},
      volume       = {23},
      number       = {1},
      issn         = {1471-2407},
      address      = {Heidelberg},
      publisher    = {Springer},
      reportid     = {DKFZ-2023-01261},
      pages        = {577},
      year         = {2023},
      abstract     = {Despite their heterogeneity, the current standard
                      preoperative radiotherapy regimen for localized high-grade
                      soft tissue sarcoma (STS) follows a one fits all approach
                      for all STS subtypes. Sarcoma patient-derived
                      three-dimensional cell culture models represent an
                      innovative tool to overcome challenges in clinical research
                      enabling reproducible subtype-specific research on STS. In
                      this pilot study, we present our methodology and preliminary
                      results using STS patient-derived 3D cell cultures that were
                      exposed to different doses of photon and proton radiation.
                      Our aim was: (i) to establish a reproducible method for
                      irradiation of STS patient-derived 3D cell cultures and (ii)
                      to explore the differences in tumor cell viability of two
                      different STS subtypes exposed to increasing doses of photon
                      and proton radiation at different time points.Two
                      patient-derived cell cultures of untreated localized
                      high-grade STS (an undifferentiated pleomorphic sarcoma
                      (UPS) and a pleomorphic liposarcoma (PLS)) were exposed to a
                      single fraction of photon or proton irradiation using doses
                      of 0 Gy (sham irradiation), 2 Gy, 4 Gy, 8 Gy and 16 Gy. Cell
                      viability was measured and compared to sham irradiation at
                      two different time points (four and eight days after
                      irradiation).The proportion of viable tumor cells four days
                      after photon irradiation for UPS vs. PLS were significantly
                      different with $85\%$ vs. $65\%$ (4 Gy), $80\%$ vs. $50\%$
                      (8 Gy) and $70\%$ vs. $35\%$ (16 Gy). Proton irradiation led
                      to similar diverging viability curves between UPS vs. PLS
                      four days after irradiation with $90\%$ vs. $75\%$ (4 Gy),
                      $85\%$ vs. $45\%$ (8 Gy) and $80\%$ vs. $35\%$ (16 Gy).
                      Photon and proton radiation displayed only minor differences
                      in cell-killing properties within each cell culture (UPS and
                      PLS). The cell-killing effect of radiation sustained at
                      eight days after irradiation in both cell
                      cultures.Pronounced differences in radiosensitivity are
                      evident among UPS and PLS 3D patient-derived sarcoma cell
                      cultures which may reflect the clinical heterogeneity.
                      Photon and proton radiation showed similar dose-dependent
                      cell-killing effectiveness in both 3D cell cultures.
                      Patient-derived 3D STS cell cultures may represent a
                      valuable tool to enable translational studies towards
                      individualized subtype-specific radiotherapy in patients
                      with STS.},
      keywords     = {Organoid (Other) / Patient-derived (Other) / Photon (Other)
                      / Proton (Other) / Radiotherapy (Other) / Sarcoma, 3D cell
                      culture (Other) / Soft tissue sarcoma (Other)},
      cin          = {BE01},
      ddc          = {610},
      cid          = {I:(DE-He78)BE01-20160331},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:37349697},
      doi          = {10.1186/s12885-023-11013-y},
      url          = {https://inrepo02.dkfz.de/record/277077},
}