TY - JOUR AU - Plum, Thomas AU - Binzberger, Rebecca AU - Thiele, Robin AU - Shang, Fuwei AU - Postrach, Daniel AU - Fung, Candice AU - Fortea, Marina AU - Stakenborg, Nathalie AU - Wang, Zheng AU - Tappe-Theodor, Anke AU - Poth, Tanja AU - MacLaren, Duncan AU - Boeckxstaens, Guy AU - Kuner, Rohini AU - Pitzer, Claudia AU - Monyer, Hannah AU - Xin, Cuiyan AU - Bonventre, Joseph V AU - Tanaka, Satoshi AU - Voehringer, David AU - Berghe, Pieter Vanden AU - Strid, Jessica AU - Feyerabend, Thorsten AU - Rodewald, Hans-Reimer TI - Mast cells link immune sensing to antigen-avoidance behaviour. JO - Nature VL - 620 IS - 7974 SN - 0028-0836 CY - London [u.a.] PB - Nature Publ. Group M1 - DKFZ-2023-01397 SP - 634-642 PY - 2023 N1 - #EA:D110#LA:D110# / 2023 Aug;620(7974):634-642 AB - The physiological functions of mast cells remain largely an enigma. In the context of barrier damage, mast cells are integrated in type 2 immunity and, together with immunoglobulin E (IgE), promote allergic diseases. Allergic symptoms may, however, facilitate expulsion of allergens, toxins and parasites and trigger future antigen avoidance1-3. Here, we show that antigen-specific avoidance behaviour in inbred mice4,5 is critically dependent on mast cells; hence, we identify the immunological sensor cell linking antigen recognition to avoidance behaviour. Avoidance prevented antigen-driven adaptive, innate and mucosal immune activation and inflammation in the stomach and small intestine. Avoidance was IgE dependent, promoted by Th2 cytokines in the immunization phase and by IgE in the execution phase. Mucosal mast cells lining the stomach and small intestine rapidly sensed antigen ingestion. We interrogated potential signalling routes between mast cells and the brain using mutant mice, pharmacological inhibition, neural activity recordings and vagotomy. Inhibition of leukotriene synthesis impaired avoidance, but overall no single pathway interruption completely abrogated avoidance, indicating complex regulation. Collectively, the stage for antigen avoidance is set when adaptive immunity equips mast cells with IgE as a telltale of past immune responses. On subsequent antigen ingestion, mast cells signal termination of antigen intake. Prevention of immunopathology-causing, continuous and futile responses against per se innocuous antigens or of repeated ingestion of toxins through mast-cell-mediated antigen-avoidance behaviour may be an important arm of immunity. LB - PUB:(DE-HGF)16 C6 - pmid:37438525 DO - DOI:10.1038/s41586-023-06188-0 UR - https://inrepo02.dkfz.de/record/277476 ER -