% IMPORTANT: The following is UTF-8 encoded. This means that in the presence % of non-ASCII characters, it will not work with BibTeX 0.99 or older. % Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or % “biber”. @ARTICLE{Placke:282517, author = {J.-M. Placke$^*$ and M. Kimmig and K. Griewank and R. Herbst and P. Terheyden and J. Utikal$^*$ and C. Pföhler and J. Ulrich and A. Kreuter and P. Mohr and R. Gutzmer and F. Meier and E. Dippel and J. Welzel and D. R. Engel$^*$ and S. Kreft and A. Sucker and G. Lodde and F. Krefting and I. Stoffels and J. Klode and A. Roesch$^*$ and L. Zimmer and E. Livingstone and E. Hadaschik and J. Becker$^*$ and M. Weichenthal and A. Tasdogan$^*$ and D. Schadendorf$^*$ and S. Ugurel$^*$}, title = {{C}orrelation of tumor {PD}-{L}1 expression in different tissue types and outcome of {PD}-1-based immunotherapy in metastatic melanoma - analysis of the {D}e{COG} prospective multicenter cohort study {ADOREG}/{TRIM}.}, journal = {EBioMedicine}, volume = {96}, issn = {2352-3964}, address = {Amsterdam [u.a.]}, publisher = {Elsevier}, reportid = {DKFZ-2023-01806}, pages = {104774}, year = {2023}, abstract = {PD-1-based immune checkpoint inhibition (ICI) is the major backbone of current melanoma therapy. Tumor PD-L1 expression represents one of few biomarkers predicting ICI therapy outcome. The objective of the present study was to systematically investigate whether the type of tumor tissue examined for PD-L1 expression has an impact on the correlation with ICI therapy outcome.Pre-treatment tumor tissue was collected within the prospective DeCOG cohort study ADOREG/TRIM (CA209-578; NCT05750511) between February 2014 and May 2020 from 448 consecutive patients who received PD-1-based ICI for non-resectable metastatic melanoma. The primary study endpoint was best overall response (BOR), secondary endpoints were progression-free (PFS) and overall survival (OS). All endpoints were correlated with tumor PD-L1 expression (quantified with clone 28-8; cutoff $≥5\%)$ and stratified by tissue type.Tumor PD-L1 was determined in 95 primary tumors (PT; $36.8\%$ positivity), 153 skin/subcutaneous $(34.0\%$ positivity), 115 lymph node (LN; $50.4\%$ positivity), and 85 organ $(40.8\%$ positivity) metastases. Tumor PD-L1 correlated with BOR if determined in LN (OR = 0.319; $95\%$ CI = 0.138-0.762; P = 0.010), but not in skin/subcutaneous metastases (OR = 0.656; $95\%$ CI = 0.311-1.341; P = 0.26). PD-L1 positivity determined on LN metastases was associated with favorable survival (PFS, HR = 0.490; $95\%$ CI = 0.310-0.775; P = 0.002; OS, HR = 0.519; $95\%$ CI = 0.307-0.880; P = 0.014). PD-L1 positivity determined in PT (PFS, HR = 0.757; $95\%$ CI = 0.467-1.226; P = 0.27; OS; HR = 0.528; $95\%$ CI = 0.305-0.913; P = 0.032) was correlated with survival to a lesser extent. No relevant survival differences were detected by PD-L1 determined in skin/subcutaneous metastases (PFS, HR = 0.825; $95\%$ CI = 0.555-1.226; P = 0.35; OS, HR = 1.083; $95\%$ CI = 0.698-1.681; P = 0.72).For PD-1-based immunotherapy in melanoma, tumor PD-L1 determined in LN metastases was stronger correlated with therapy outcome than that assessed in PT or organ metastases. PD-L1 determined in skin/subcutaneous metastases showed no outcome correlation and therefore should be used with caution for clinical decision making.Bristol-Myers Squibb (ADOREG/TRIM, NCT05750511); German Research Foundation (DFG; Clinician Scientist Program UMEA); Else Kröner-Fresenius-Stiftung (EKFS; Medical Scientist Academy UMESciA).}, keywords = {Biomarker (Other) / Immune checkpoint inhibition (Other) / Melanoma (Other) / Therapy outcome (Other) / Tumor PD-L1 (Other)}, cin = {A370 / ED01}, ddc = {610}, cid = {I:(DE-He78)A370-20160331 / I:(DE-He78)ED01-20160331}, pnm = {311 - Zellbiologie und Tumorbiologie (POF4-311)}, pid = {G:(DE-HGF)POF4-311}, typ = {PUB:(DE-HGF)16}, pubmed = {pmid:37660535}, doi = {10.1016/j.ebiom.2023.104774}, url = {https://inrepo02.dkfz.de/record/282517}, }