TY  - JOUR
AU  - Yayli, Gizem
AU  - Bernardini, Andrea
AU  - Mendoza Sanchez, Paulina Karen
AU  - Scheer, Elisabeth
AU  - Damilot, Mylène
AU  - Essabri, Karim
AU  - Morlet, Bastien
AU  - Negroni, Luc
AU  - Vincent, Stéphane D
AU  - Timmers, H T Marc
AU  - Tora, László
TI  - ATAC and SAGA co-activator complexes utilize co-translational assembly, but their cellular localization properties and functions are distinct.
JO  - Cell reports
VL  - 42
IS  - 9
SN  - 2211-1247
CY  - [New York, NY]
PB  - Elsevier
M1  - DKFZ-2023-01842
SP  - 113099
PY  - 2023
AB  - To understand the function of multisubunit complexes, it is of key importance to uncover the precise mechanisms that guide their assembly. Nascent proteins can find and bind their interaction partners during their translation, leading to co-translational assembly. Here, we demonstrate that the core modules of ATAC (ADA-two-A-containing) and SAGA (Spt-Ada-Gcn5-acetyltransferase), two lysine acetyl transferase-containing transcription co-activator complexes, assemble co-translationally in the cytoplasm of mammalian cells. In addition, a SAGA complex containing all of its modules forms in the cytoplasm and acetylates non-histone proteins. In contrast, ATAC complex subunits cannot be detected in the cytoplasm of mammalian cells. However, an endogenous ATAC complex containing two functional modules forms and functions in the nucleus. Thus, the two related co-activators, ATAC and SAGA, assemble using co-translational pathways, but their subcellular localization, cytoplasmic abundance, and functions are distinct.
KW  - CP: Molecular biology (Other)
KW  - RIP (Other)
KW  - RNA immunoprecipitation (Other)
KW  - YEATS2 (Other)
KW  - ZZZ3 (Other)
KW  - biogenesis (Other)
KW  - co-translation (Other)
KW  - lysine acetylation (Other)
KW  - multiprotein complex (Other)
KW  - non-histone protein (Other)
KW  - ribosome (Other)
KW  - single-molecule RNA FISH (Other)
KW  - transcription regulation (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:37682711
DO  - DOI:10.1016/j.celrep.2023.113099
UR  - https://inrepo02.dkfz.de/record/282716
ER  -