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@ARTICLE{Yayli:282716,
author = {G. Yayli and A. Bernardini and P. K. Mendoza Sanchez$^*$
and E. Scheer and M. Damilot and K. Essabri and B. Morlet
and L. Negroni and S. D. Vincent and H. T. M. Timmers$^*$
and L. Tora},
title = {{ATAC} and {SAGA} co-activator complexes utilize
co-translational assembly, but their cellular localization
properties and functions are distinct.},
journal = {Cell reports},
volume = {42},
number = {9},
issn = {2211-1247},
address = {[New York, NY]},
publisher = {Elsevier},
reportid = {DKFZ-2023-01842},
pages = {113099},
year = {2023},
abstract = {To understand the function of multisubunit complexes, it is
of key importance to uncover the precise mechanisms that
guide their assembly. Nascent proteins can find and bind
their interaction partners during their translation, leading
to co-translational assembly. Here, we demonstrate that the
core modules of ATAC (ADA-two-A-containing) and SAGA
(Spt-Ada-Gcn5-acetyltransferase), two lysine acetyl
transferase-containing transcription co-activator complexes,
assemble co-translationally in the cytoplasm of mammalian
cells. In addition, a SAGA complex containing all of its
modules forms in the cytoplasm and acetylates non-histone
proteins. In contrast, ATAC complex subunits cannot be
detected in the cytoplasm of mammalian cells. However, an
endogenous ATAC complex containing two functional modules
forms and functions in the nucleus. Thus, the two related
co-activators, ATAC and SAGA, assemble using
co-translational pathways, but their subcellular
localization, cytoplasmic abundance, and functions are
distinct.},
keywords = {CP: Molecular biology (Other) / RIP (Other) / RNA
immunoprecipitation (Other) / YEATS2 (Other) / ZZZ3 (Other)
/ biogenesis (Other) / co-translation (Other) / lysine
acetylation (Other) / multiprotein complex (Other) /
non-histone protein (Other) / ribosome (Other) /
single-molecule RNA FISH (Other) / transcription regulation
(Other)},
cin = {FR01},
ddc = {610},
cid = {I:(DE-He78)FR01-20160331},
pnm = {899 - ohne Topic (POF4-899)},
pid = {G:(DE-HGF)POF4-899},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:37682711},
doi = {10.1016/j.celrep.2023.113099},
url = {https://inrepo02.dkfz.de/record/282716},
}