% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Welters:283180,
      author       = {C. Welters and M.-L. Welters and S. Stadler$^*$ and L.
                      Bullinger$^*$ and J. Strobel and H. Hackstein and A.
                      Dhamodaran and T. Blankenstein and L. A. Hansmann$^*$},
      title        = {{HLA}-{C}*04:09{N} is expressed at the cell surface and
                      triggers peptide-specific {T}-cell activation.},
      journal      = {Haematologica},
      volume       = {109},
      number       = {4},
      issn         = {0390-6078},
      address      = {Pavia},
      publisher    = {Ferrata Storti Foundation},
      reportid     = {DKFZ-2023-01959},
      pages        = {1121-1127},
      year         = {2024},
      note         = {2024 Apr 1;109(4):1121-1127},
      abstract     = {The null allele HLA-C*04:09N differs from HLA-C*04:01 in a
                      frameshift mutation within its cytoplasmic domain, resulting
                      in translation of 32 additional amino acids that are assumed
                      to prevent cell surface expression. However, we recently
                      identified a multiple myeloma-reactive T-cell receptor (TCR)
                      that appeared to recognize antigen presented on HLA-C*04:09N
                      and encouraged us to ask whether HLA-C*04:09N, albeit not
                      easily detectable at the cell surface, can present antigen
                      sufficient for T-cell activation. We generated two HLA-class
                      I-deficient cell lines, re-expressed HLA-C*04:09N, detected
                      HLA expression by flow cytometry, and tested for T-cell
                      activation using a cytomegalovirus peptide-specific
                      HLA-C*04:01-restricted TCR. In both cell lines, HLA-C*04:09N
                      expression was detectable at the cell surface and could be
                      enhanced by IFN-γ exposure. Recombinant HLA-C*04:09N
                      expression was sufficient for T-cell activation in vitro,
                      which could be blocked by an HLA-class I-specific antibody,
                      suggesting HLA-TCR interaction at the cell surface.
                      Peripheral blood mononuclear cells isolated from an
                      individual who physiologically expressed HLA-C*04:09N
                      triggered peptide-specific T-cell activation, confirming our
                      results with cells with natural HLA expression levels. In
                      conclusion, we present peptide-specific
                      HLA-C*04:09N-restricted T-cell activation and suggest
                      consideration of this allele in the appropriate clinical
                      context such as allogeneic stem cell transplantation, or in
                      the setting of cellular therapy.},
      cin          = {BE01},
      ddc          = {610},
      cid          = {I:(DE-He78)BE01-20160331},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:37767552},
      doi          = {10.3324/haematol.2023.283812},
      url          = {https://inrepo02.dkfz.de/record/283180},
}