TY  - JOUR
AU  - Blanco-Carmona, Enrique
AU  - Narayanan, Ashwin
AU  - Hernandez, Inmaculada
AU  - Nieto, Juan C
AU  - Elosua-Bayes, Marc
AU  - Sun, Xueyuan
AU  - Schmidt, Claudia
AU  - Pamir, Necmettin
AU  - Özduman, Koray
AU  - Herold-Mende, Christel
AU  - Pagani, Francesca
AU  - Cominelli, Manuela
AU  - Taranda, Julian
AU  - Wick, Wolfgang
AU  - von Deimling, Andreas
AU  - Poliani, Pietro Luigi
AU  - Rehli, Michael
AU  - Schlesner, Matthias
AU  - Heyn, Holger
AU  - Turcan, Sevin
TI  - Tumor heterogeneity and tumor-microglia interactions in primary and recurrent IDH1-mutant gliomas.
JO  - Cell reports / Medicine
VL  - 4
IS  - 11
SN  - 2666-3791
CY  - Maryland Heights, MO
PB  - Elsevier
M1  - DKFZ-2023-02178
SP  - 101249
PY  - 2023
N1  - #EA:B062#LA:B320# / 2023 Nov 21;4(11):101249
AB  - The isocitrate dehydrogenase (IDH) gene is recurrently mutated in adult diffuse gliomas. IDH-mutant gliomas are categorized into oligodendrogliomas and astrocytomas, each with unique pathological features. Here, we use single-nucleus RNA and ATAC sequencing to compare the molecular heterogeneity of these glioma subtypes. In addition to astrocyte-like, oligodendrocyte progenitor-like, and cycling tumor subpopulations, a tumor population enriched for ribosomal genes and translation elongation factors is primarily present in oligodendrogliomas. Longitudinal analysis of astrocytomas indicates that the proportion of tumor subpopulations remains stable in recurrent tumors. Analysis of tumor-associated microglia/macrophages (TAMs) reveals significant differences between oligodendrogliomas, with astrocytomas harboring inflammatory TAMs expressing phosphorylated STAT1, as confirmed by immunohistochemistry. Furthermore, inferred receptor-ligand interactions between tumor subpopulations and TAMs may contribute to TAM state diversity. Overall, our study sheds light on distinct tumor populations, TAM heterogeneity, TAM-tumor interactions in IDH-mutant glioma subtypes, and the relative stability of tumor subpopulations in recurrent astrocytomas.
KW  - IDH mutation (Other)
KW  - astrocytoma (Other)
KW  - bone-marrow-derived macrophages (Other)
KW  - glioma (Other)
KW  - microglia (Other)
KW  - oligodendroglioma (Other)
KW  - recurrent glioma (Other)
KW  - snATAC-seq (Other)
KW  - snRNA-seq (Other)
KW  - tumor microenvironment (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:37883975
DO  - DOI:10.1016/j.xcrm.2023.101249
UR  - https://inrepo02.dkfz.de/record/285005
ER  -