%0 Journal Article
%A Selt, Florian
%A El Damaty, Ahmed
%A Schuhmann, Martin U
%A Sigaud, Romain
%A Ecker, Jonas
%A Sievers, Philipp
%A Kocher, Daniela
%A Herold-Mende, Christel
%A Oehme, Ina
%A von Deimling, Andreas
%A Pfister, Stefan
%A Sahm, Felix
%A Jones, David
%A Witt, Olaf
%A Milde, Till
%T Generation of patient-derived pediatric pilocytic astrocytoma in-vitro models using SV40 large T: evaluation of a modeling workflow.
%J Journal of neuro-oncology
%V 165
%N 3
%@ 0167-594X
%C Dordrecht [u.a.]
%I Springer Science + Business Media B.V
%M DKFZ-2023-02478
%P 467-478
%D 2023
%Z #EA:B310#LA:B310# / 2023 Dec;165(3):467-478
%X Although pediatric low-grade gliomas (pLGG) are the most common pediatric brain tumors, patient-derived cell lines reflecting pLGG biology in culture are scarce. This also applies to the most common pLGG subtype pilocytic astrocytoma (PA). Conventional cell culture approaches adapted from higher-grade tumors fail in PA due to oncogene-induced senescence (OIS) driving tumor cells into arrest. Here, we describe a PA modeling workflow using the Simian Virus large T antigen (SV40-TAg) to circumvent OIS.18 pLGG tissue samples (17 (94
%K Circumvention of OIS (Other)
%K In-vitro models (Other)
%K Inducible SV40 large T (Other)
%K Pediatric low-grade glioma cell lines (Other)
%K Pilocytic astrocytoma (Other)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:37999877
%R 10.1007/s11060-023-04500-6
%U https://inrepo02.dkfz.de/record/285655