TY  - JOUR
AU  - Selt, Florian
AU  - El Damaty, Ahmed
AU  - Schuhmann, Martin U
AU  - Sigaud, Romain
AU  - Ecker, Jonas
AU  - Sievers, Philipp
AU  - Kocher, Daniela
AU  - Herold-Mende, Christel
AU  - Oehme, Ina
AU  - von Deimling, Andreas
AU  - Pfister, Stefan
AU  - Sahm, Felix
AU  - Jones, David
AU  - Witt, Olaf
AU  - Milde, Till
TI  - Generation of patient-derived pediatric pilocytic astrocytoma in-vitro models using SV40 large T: evaluation of a modeling workflow.
JO  - Journal of neuro-oncology
VL  - 165
IS  - 3
SN  - 0167-594X
CY  - Dordrecht [u.a.]
PB  - Springer Science + Business Media B.V
M1  - DKFZ-2023-02478
SP  - 467-478
PY  - 2023
N1  - #EA:B310#LA:B310# / 2023 Dec;165(3):467-478
AB  - Although pediatric low-grade gliomas (pLGG) are the most common pediatric brain tumors, patient-derived cell lines reflecting pLGG biology in culture are scarce. This also applies to the most common pLGG subtype pilocytic astrocytoma (PA). Conventional cell culture approaches adapted from higher-grade tumors fail in PA due to oncogene-induced senescence (OIS) driving tumor cells into arrest. Here, we describe a PA modeling workflow using the Simian Virus large T antigen (SV40-TAg) to circumvent OIS.18 pLGG tissue samples (17 (94
KW  - Circumvention of OIS (Other)
KW  - In-vitro models (Other)
KW  - Inducible SV40 large T (Other)
KW  - Pediatric low-grade glioma cell lines (Other)
KW  - Pilocytic astrocytoma (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:37999877
DO  - DOI:10.1007/s11060-023-04500-6
UR  - https://inrepo02.dkfz.de/record/285655
ER  -