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@ARTICLE{AlonsodelaVega:285670,
author = {A. Alonso de la Vega$^*$ and N. A. Temiz and R. Tasakis$^*$
and K. Somogyi$^*$ and L. Salgueiro$^*$ and E. Zimmer$^*$
and M. Ramos$^*$ and A. Diaz-Jimenez$^*$ and S. Chocarro$^*$
and M. Fernández-Vaquero$^*$ and B. Stefanovska and E.
Reuveni and U. Ben-David and A. Stenzinger and T. Poth and
M. Heikenwälder$^*$ and N. Papavasiliou$^*$ and R. S.
Harris and R. Sotillo$^*$},
title = {{A}cute expression of human {APOBEC}3{B} in mice results in
{RNA} editing and lethality.},
journal = {Genome biology},
volume = {24},
number = {1},
issn = {1465-6906},
address = {London},
publisher = {BioMed Central},
reportid = {DKFZ-2023-02493},
pages = {267},
year = {2023},
note = {#EA:B220#LA:B220#},
abstract = {RNA editing has been described as promoting genetic
heterogeneity, leading to the development of multiple
disorders, including cancer. The cytosine deaminase APOBEC3B
is implicated in tumor evolution through DNA mutation, but
whether it also functions as an RNA editing enzyme has not
been studied.Here, we engineer a novel doxycycline-inducible
mouse model of human APOBEC3B-overexpression to understand
the impact of this enzyme in tissue homeostasis and address
a potential role in C-to-U RNA editing. Elevated and
sustained levels of APOBEC3B lead to rapid alteration of
cellular fitness, major organ dysfunction, and ultimately
lethality in mice. Importantly, RNA-sequencing of mouse
tissues expressing high levels of APOBEC3B identifies
frequent UCC-to-UUC RNA editing events that are not evident
in the corresponding genomic DNA.This work identifies, for
the first time, a new deaminase-dependent function for
APOBEC3B in RNA editing and presents a preclinical tool to
help understand the emerging role of APOBEC3B as a driver of
carcinogenesis.},
keywords = {Humans / Animals / Mice / RNA Editing / Cytidine Deaminase:
genetics / Cytidine Deaminase: metabolism / Mutation /
Neoplasms: pathology / Minor Histocompatibility Antigens:
genetics / Minor Histocompatibility Antigens: metabolism /
DNA: metabolism / APOBEC3B (Other) / DNA damage (Other) /
Mouse models (Other) / Mutations (Other) / RNA editing
(Other) / Cytidine Deaminase (NLM Chemicals) / Minor
Histocompatibility Antigens (NLM Chemicals) / DNA (NLM
Chemicals) / APOBEC3B protein, human (NLM Chemicals)},
cin = {B220 / F180 / D150},
ddc = {570},
cid = {I:(DE-He78)B220-20160331 / I:(DE-He78)F180-20160331 /
I:(DE-He78)D150-20160331},
pnm = {312 - Funktionelle und strukturelle Genomforschung
(POF4-312)},
pid = {G:(DE-HGF)POF4-312},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:38001542},
pmc = {pmc:PMC10668425},
doi = {10.1186/s13059-023-03115-4},
url = {https://inrepo02.dkfz.de/record/285670},
}