%0 Journal Article
%A Emde-Rajaratnam, Martina
%A Beck, Susanne
%A Benes, Vladimir
%A Salwender, Hans
%A Bertsch, Uta
%A Scheid, Christoph
%A Hänel, Mathias
%A Weisel, Katja
%A Hielscher, Thomas
%A Raab, Marc S
%A Goldschmidt, Hartmut
%A Jauch, Anna
%A Maes, Ken
%A De Bruyne, Elke
%A Menu, Eline
%A De Veirman, Kim
%A Moreaux, Jérôme
%A Vanderkerken, Karin
%A Seckinger, Anja
%A Hose, Dirk
%T RNA-sequencing based first choice of treatment and determination of risk in multiple myeloma.
%J Frontiers in immunology
%V 14
%@ 1664-3224
%C Lausanne
%I Frontiers Media
%M DKFZ-2023-02521
%P 1286700
%D 2023
%X Immunotherapeutic targets in multiple myeloma (MM) have variable expression height and are partly expressed in subfractions of patients only. With increasing numbers of available compounds, strategies for appropriate choice of targets (combinations) are warranted. Simultaneously, risk assessment is advisable as patient's life expectancy varies between months and decades.We first assess feasibility of RNA-sequencing in a multicenter trial (GMMG-MM5, n=604 patients). Next, we use a clinical routine cohort of untreated symptomatic myeloma patients undergoing autologous stem cell transplantation (n=535, median follow-up (FU) 64 months) to perform RNA-sequencing, gene expression profiling (GEP), and iFISH by ten-probe panel on CD138-purified malignant plasma cells. We subsequently compare target expression to plasma cell precursors, MGUS (n=59), asymptomatic (n=142) and relapsed (n=69) myeloma patients, myeloma cell lines (n=26), and between longitudinal samples (MM vs. relapsed MM). Data are validated using the independent MMRF CoMMpass-cohort (n=767, FU 31 months).RNA-sequencing is feasible in 90.8
%K RNA-sequencing (Other)
%K immunotherapeutic targets (Other)
%K multiple myeloma (Other)
%K personalized treatment (Other)
%K proliferation (Other)
%K risk-adapted treatment (Other)
%K survival (Other)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:38035078
%2 pmc:PMC10684778
%R 10.3389/fimmu.2023.1286700
%U https://inrepo02.dkfz.de/record/285713