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000286010 1001_ $$00000-0001-6149-345X$$aLucien, Fabrice$$b0
000286010 245__ $$aMIBlood-EV: Minimal information to enhance the quality and reproducibility of blood extracellular vesicle research.
000286010 260__ $$aHoboken, NJ$$bWiley$$c2023
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000286010 520__ $$aBlood is the most commonly used body fluid for extracellular vesicle (EV) research. The composition of a blood sample and its derivatives (i.e., plasma and serum) are not only donor-dependent but also influenced by collection and preparation protocols. Since there are hundreds of pre-analytical protocols and over forty variables, the development of standard operating procedures for EV research is very challenging. To improve the reproducibility of blood EV research, the International Society for Extracellular Vesicles (ISEV) Blood EV Task Force proposes standardized reporting of (i) the applied blood collection and preparation protocol and (ii) the quality of the prepared plasma and serum samples. Gathering detailed information will provide insight into the performance of the protocols and more effectively identify potential confounders in the prepared plasma and serum samples. To collect this information, the ISEV Blood EV Task Force created the Minimal Information for Blood EV research (MIBlood-EV), a tool to record and report information about pre-analytical protocols used for plasma and serum preparation as well as assays used to assess the quality of these preparations. This tool does not require modifications of established local pre-analytical protocols and can be easily implemented to enhance existing databases thereby enabling evidence-based optimization of pre-analytical protocols through meta-analysis. Taken together, insight into the quality of prepared plasma and serum samples will (i) improve the quality of biobanks for EV research, (ii) guide the exchange of plasma and serum samples between biobanks and laboratories, (iii) facilitate inter-laboratory comparative EV studies, and (iv) improve the peer review process.
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000286010 650_7 $$2Other$$abiomarker
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000286010 650_7 $$2Other$$aextracellular vesicles
000286010 650_7 $$2Other$$aliquid biopsy
000286010 650_7 $$2Other$$aquality control
000286010 650_7 $$2Other$$areproducibility
000286010 650_7 $$2Other$$astandardization
000286010 7001_ $$aGustafson, Dakota$$b1
000286010 7001_ $$00000-0002-9488-6855$$aLenassi, Metka$$b2
000286010 7001_ $$aLi, Bo$$b3
000286010 7001_ $$aTeske, Jacob J$$b4
000286010 7001_ $$aBoilard, Eric$$b5
000286010 7001_ $$0P:(DE-HGF)0$$avon Hohenberg, Katharina Clemm$$b6
000286010 7001_ $$00000-0003-3133-0670$$aFalcón-Perez, Juan Manual$$b7
000286010 7001_ $$00000-0003-2996-5714$$aGualerzi, Alice$$b8
000286010 7001_ $$aReale, Antonia$$b9
000286010 7001_ $$aJones, Jennifer C$$b10
000286010 7001_ $$aLässer, Cecilia$$b11
000286010 7001_ $$aLawson, Charlotte$$b12
000286010 7001_ $$00000-0002-2633-1161$$aNazarenko, Irina$$b13
000286010 7001_ $$aO'Driscoll, Lorraine$$b14
000286010 7001_ $$00000-0001-7501-558X$$aPink, Ryan$$b15
000286010 7001_ $$00000-0003-2326-5821$$aSiljander, Pia R-M$$b16
000286010 7001_ $$aSoekmadji, Carolina$$b17
000286010 7001_ $$aHendrix, An$$b18
000286010 7001_ $$00000-0002-1097-9756$$aWelsh, Joshua A$$b19
000286010 7001_ $$00000-0003-1664-4233$$aWitwer, Kenneth W$$b20
000286010 7001_ $$00000-0002-5671-3400$$aNieuwland, Rienk$$b21
000286010 773__ $$0PERI:(DE-600)2683797-3$$a10.1002/jev2.12385$$gVol. 12, no. 12, p. 12385$$n12$$p12385$$tJournal of extracellular vesicles$$v12$$x2001-3078$$y2023
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