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000286587 1001_ $$aSichero, Laura$$b0
000286587 245__ $$aDetection of serum biomarkers of HPV-16 driven oropharynx and oral cavity cancer in Brazil.
000286587 260__ $$aAmsterdam [u.a.]$$bElsevier Science$$c2024
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000286587 520__ $$aHPV-16 driven oropharynx/oral cavity squamous cell carcinomas prevalence varies globally. We evaluated the presence of HPV-16 ctDNA and HPV-16 E6 antibodies in samples obtained from participants treated at the Instituto do Cancer do Estado de Sao Paulo, ICESP, and from whom tumoral HPV DNA, HPV-16 E6*I mRNA, and p16INK4a status was also accessed.HPV was genotyped by PCR-hybridization. All HPV DNA positive and ∼10 % HPV DNA negative cases underwent p16INK4a immunohistochemistry and E6*I RNA testing using a multiplex bead based protocol. HPV-16 ctDNA and anti-E6 antibodies were assessed by ddPCR (digital droplet PCR) and multiplex serology, respectively.The prevalence of HPV-16 in oropharynx carcinoma (OPC) cases was low (8.7 %) when considering solely HPV-16 DNA detection, and even lower (5.2 %) when taken into consideration the concomitant detection of HPV-16 E6*I RNA and/or p16INK4 (HPV-16 attributable fraction - AF). None of the oral cavity cancer (OCC) cases were detected with HPV-16 DNA. HPV-16 ctDNA was more commonly detected than HPV-16 E6 antibodies (29.8 % versus 10.6 %). Both serum biomarkers attained 100 % sensitivity of detecting HPV-16 AF OPC, however the specificity of the HPV-16 anti-E6 biomarker was higher compared to ctDNA (93.2 % versus 75.0 %). Finally, when both HPV-16 ctDNA and anti-E6 biomarkers were considered together, the sensitivity and specificity for HPV-16 OPC detection was 100 % and about 70 %, respectively, independently of analyzing HPV-16 DNA positive or HPV-16 AF tumors.Our findings corroborate that serum biomarkers are highly sensitive and specific biomarkers for detection of HPV-associated OPC.
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000286587 650_7 $$2Other$$aAntibodies
000286587 650_7 $$2Other$$aHPV ctDNA
000286587 650_7 $$2Other$$aHead and neck cancer
000286587 650_7 $$2Other$$aHuman papillomavirus
000286587 650_7 $$2Other$$aSerum biomarkers
000286587 7001_ $$aGonçalves, Milena Giulia$$b1
000286587 7001_ $$aBettoni, Fabiana$$b2
000286587 7001_ $$aCoser, Elisangela Monteiro$$b3
000286587 7001_ $$aMota, Giana$$b4
000286587 7001_ $$aNunes, Rafaella A L$$b5
000286587 7001_ $$aMercante, Ana Maria da Cunha$$b6
000286587 7001_ $$aNatalino, Renato$$b7
000286587 7001_ $$aUno, Miyuki$$b8
000286587 7001_ $$aFerreira Alves, Maria José$$b9
000286587 7001_ $$aMatos, Leandro Luongo$$b10
000286587 7001_ $$aKowalski, Luiz Paulo$$b11
000286587 7001_ $$aKulcsar, Marco Aurélio Vamondes$$b12
000286587 7001_ $$ade Alvarenga, Gustavo Fernandes$$b13
000286587 7001_ $$0P:(DE-He78)3b05a05c74fcfafec06747c40ac929c0$$aHöfler, Daniela$$b14$$udkfz
000286587 7001_ $$0P:(DE-He78)b12aaacaa142dabed85c6d84d00bf0fb$$aSchroeder, Lea$$b15$$udkfz
000286587 7001_ $$0P:(DE-He78)6b4ebb9791b983b5620c0caaf3468e30$$aWaterboer, Tim$$b16$$udkfz
000286587 7001_ $$aTommasino, Massimo$$b17
000286587 7001_ $$aVilla, Luisa Lina$$b18
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