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@ARTICLE{Sichero:286587,
      author       = {L. Sichero and M. G. Gonçalves and F. Bettoni and E. M.
                      Coser and G. Mota and R. A. L. Nunes and A. M. d. C.
                      Mercante and R. Natalino and M. Uno and M. J. Ferreira Alves
                      and L. L. Matos and L. P. Kowalski and M. A. V. Kulcsar and
                      G. F. de Alvarenga and D. Höfler$^*$ and L. Schroeder$^*$
                      and T. Waterboer$^*$ and M. Tommasino and L. L. Villa},
      title        = {{D}etection of serum biomarkers of {HPV}-16 driven
                      oropharynx and oral cavity cancer in {B}razil.},
      journal      = {Oral oncology},
      volume       = {149},
      issn         = {0964-1955},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier Science},
      reportid     = {DKFZ-2023-02804},
      pages        = {106676},
      year         = {2024},
      note         = {Volume 149, February 2024, 106676},
      abstract     = {HPV-16 driven oropharynx/oral cavity squamous cell
                      carcinomas prevalence varies globally. We evaluated the
                      presence of HPV-16 ctDNA and HPV-16 E6 antibodies in samples
                      obtained from participants treated at the Instituto do
                      Cancer do Estado de Sao Paulo, ICESP, and from whom tumoral
                      HPV DNA, HPV-16 E6*I mRNA, and p16INK4a status was also
                      accessed.HPV was genotyped by PCR-hybridization. All HPV DNA
                      positive and ∼10 $\%$ HPV DNA negative cases underwent
                      p16INK4a immunohistochemistry and E6*I RNA testing using a
                      multiplex bead based protocol. HPV-16 ctDNA and anti-E6
                      antibodies were assessed by ddPCR (digital droplet PCR) and
                      multiplex serology, respectively.The prevalence of HPV-16 in
                      oropharynx carcinoma (OPC) cases was low (8.7 $\%)$ when
                      considering solely HPV-16 DNA detection, and even lower (5.2
                      $\%)$ when taken into consideration the concomitant
                      detection of HPV-16 E6*I RNA and/or p16INK4 (HPV-16
                      attributable fraction - AF). None of the oral cavity cancer
                      (OCC) cases were detected with HPV-16 DNA. HPV-16 ctDNA was
                      more commonly detected than HPV-16 E6 antibodies (29.8 $\%$
                      versus 10.6 $\%).$ Both serum biomarkers attained 100 $\%$
                      sensitivity of detecting HPV-16 AF OPC, however the
                      specificity of the HPV-16 anti-E6 biomarker was higher
                      compared to ctDNA (93.2 $\%$ versus 75.0 $\%).$ Finally,
                      when both HPV-16 ctDNA and anti-E6 biomarkers were
                      considered together, the sensitivity and specificity for
                      HPV-16 OPC detection was 100 $\%$ and about 70 $\%,$
                      respectively, independently of analyzing HPV-16 DNA positive
                      or HPV-16 AF tumors.Our findings corroborate that serum
                      biomarkers are highly sensitive and specific biomarkers for
                      detection of HPV-associated OPC.},
      keywords     = {Antibodies (Other) / HPV ctDNA (Other) / Head and neck
                      cancer (Other) / Human papillomavirus (Other) / Serum
                      biomarkers (Other)},
      cin          = {F020},
      ddc          = {610},
      cid          = {I:(DE-He78)F020-20160331},
      pnm          = {316 - Infektionen, Entzündung und Krebs (POF4-316)},
      pid          = {G:(DE-HGF)POF4-316},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:38150987},
      doi          = {10.1016/j.oraloncology.2023.106676},
      url          = {https://inrepo02.dkfz.de/record/286587},
}