%0 Journal Article
%A Montero-Vergara, Jetsy
%A Plachetta, Kira
%A Kinch, Lisa
%A Bernhardt, Stephan
%A Kashyap, Kriti
%A Levine, Beth
%A Thukral, Lipi
%A Vetter, Martina
%A Thomssen, Christoph
%A Wiemann, Stefan
%A Peña-Llopis, Samuel
%A Jendrossek, Verena
%A Vega-Rubin-de-Celis, Silvia
%T GRB2 is a BECN1 interacting protein that regulates autophagy.
%J Cell death & disease
%V 15
%N 1
%@ 2041-4889
%C London [u.a.]
%I Nature Publishing Group
%M DKFZ-2024-00053
%P 14
%D 2024
%X GRB2 is an adaptor protein of HER2 (and several other tyrosine kinases), which we identified as a novel BECN1 (Beclin 1) interacting partner. GRB2 co-immunoprecipitated with BECN1 in several breast cancer cell lines and regulates autophagy through a mechanism involving the modulation of the class III PI3Kinase VPS34 activity. In ovo studies in a CAM (Chicken Chorioallantoic Membrane) model indicated that GRB2 knockdown, as well as overexpression of GRB2 loss-of-function mutants (Y52A and S86A-R88A) compromised tumor growth. These differences in tumor growth correlated with differential autophagy activity, indicating that autophagy effects might be related to the effects on tumorigenesis. Our data highlight a novel function of GRB2 as a BECN1 binding protein and a regulator of autophagy.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:38182563
%2 pmc:PMC10770341
%R 10.1038/s41419-023-06387-7
%U https://inrepo02.dkfz.de/record/286713