TY  - JOUR
AU  - Montero-Vergara, Jetsy
AU  - Plachetta, Kira
AU  - Kinch, Lisa
AU  - Bernhardt, Stephan
AU  - Kashyap, Kriti
AU  - Levine, Beth
AU  - Thukral, Lipi
AU  - Vetter, Martina
AU  - Thomssen, Christoph
AU  - Wiemann, Stefan
AU  - Peña-Llopis, Samuel
AU  - Jendrossek, Verena
AU  - Vega-Rubin-de-Celis, Silvia
TI  - GRB2 is a BECN1 interacting protein that regulates autophagy.
JO  - Cell death & disease
VL  - 15
IS  - 1
SN  - 2041-4889
CY  - London [u.a.]
PB  - Nature Publishing Group
M1  - DKFZ-2024-00053
SP  - 14
PY  - 2024
AB  - GRB2 is an adaptor protein of HER2 (and several other tyrosine kinases), which we identified as a novel BECN1 (Beclin 1) interacting partner. GRB2 co-immunoprecipitated with BECN1 in several breast cancer cell lines and regulates autophagy through a mechanism involving the modulation of the class III PI3Kinase VPS34 activity. In ovo studies in a CAM (Chicken Chorioallantoic Membrane) model indicated that GRB2 knockdown, as well as overexpression of GRB2 loss-of-function mutants (Y52A and S86A-R88A) compromised tumor growth. These differences in tumor growth correlated with differential autophagy activity, indicating that autophagy effects might be related to the effects on tumorigenesis. Our data highlight a novel function of GRB2 as a BECN1 binding protein and a regulator of autophagy.
LB  - PUB:(DE-HGF)16
C6  - pmid:38182563
C2  - pmc:PMC10770341
DO  - DOI:10.1038/s41419-023-06387-7
UR  - https://inrepo02.dkfz.de/record/286713
ER  -