%0 Journal Article
%A Iser, Florian
%A Hinz, Felix
%A Hoffmann, Dirk Carsten Frieder
%A Grassl, Niklas
%A Güngör, Cansu
%A Meyer, Jochen
%A Dörner, Laura
%A Hofmann, Lea
%A Kelbch, Vanessa
%A Göbel, Kirsten
%A Mahmutoglu, Mustafa Ahmed
%A Vollmuth, Phillipp
%A Patel, Areeba Jamilkhan
%A Nguyen, Duy
%A Kaulen, Leon
%A Mildenberger, Iris
%A Sahm, Katharina
%A Maass, Kendra
%A Pajtler, Kristian
%A Shankar, Ganesh M
%A Weiler, Markus
%A Wildemann, Brigitte
%A Winkler, Frank
%A von Deimling, Andreas
%A Platten, Michael
%A Wick, Wolfgang
%A Sahm, Felix
%A Kessler, Tobias
%T Cerebrospinal fluid cfDNA sequencing for classification of central nervous system glioma.
%J Clinical cancer research
%V 30
%N 14
%@ 1078-0432
%C Philadelphia, Pa. [u.a.]
%I AACR
%M DKFZ-2024-00255
%P 2974-2985
%D 2024
%Z #EA:B320#LA:B320#LA:B300# / 2024 Jul 15;30(14):2974-2985
%X Primary central nervous system (CNS) gliomas can be classified by characteristic genetic alterations. In addition to solid tissue obtained via surgery or biopsy, cell-free DNA (cfDNA) from cerebrospinal fluid (CSF) is an alternative source of material for genomic analyses.We performed targeted next-generation sequencing (NGS) of CSF cfDNA in a representative cohort of 85 patients presenting at two neurooncological centers with suspicion of primary or recurrent glioma. Copy-number variation (CNV) profiles, single nucleotide variants (SNVs), and small insertions/ deletions (indels) were combined into a molecular-guided tumor classification. Comparison with the solid tumor was performed for 38 cases with matching solid tissue available.Cases were stratified into four groups: glioblastoma (n = 32), other glioma (n = 19), non-malignant (n = 17) and non-diagnostic (n = 17). We introduced a molecular-guided tumor classification, which enabled identification of tumor entities and/ or cancer specific alterations in 75.0 
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:38295147
%R 10.1158/1078-0432.CCR-23-2907
%U https://inrepo02.dkfz.de/record/287463