TY  - JOUR
AU  - Iser, Florian
AU  - Hinz, Felix
AU  - Hoffmann, Dirk Carsten Frieder
AU  - Grassl, Niklas
AU  - Güngör, Cansu
AU  - Meyer, Jochen
AU  - Dörner, Laura
AU  - Hofmann, Lea
AU  - Kelbch, Vanessa
AU  - Göbel, Kirsten
AU  - Mahmutoglu, Mustafa Ahmed
AU  - Vollmuth, Phillipp
AU  - Patel, Areeba Jamilkhan
AU  - Nguyen, Duy
AU  - Kaulen, Leon
AU  - Mildenberger, Iris
AU  - Sahm, Katharina
AU  - Maass, Kendra
AU  - Pajtler, Kristian
AU  - Shankar, Ganesh M
AU  - Weiler, Markus
AU  - Wildemann, Brigitte
AU  - Winkler, Frank
AU  - von Deimling, Andreas
AU  - Platten, Michael
AU  - Wick, Wolfgang
AU  - Sahm, Felix
AU  - Kessler, Tobias
TI  - Cerebrospinal fluid cfDNA sequencing for classification of central nervous system glioma.
JO  - Clinical cancer research
VL  - 30
IS  - 14
SN  - 1078-0432
CY  - Philadelphia, Pa. [u.a.]
PB  - AACR
M1  - DKFZ-2024-00255
SP  - 2974-2985
PY  - 2024
N1  - #EA:B320#LA:B320#LA:B300# / 2024 Jul 15;30(14):2974-2985
AB  - Primary central nervous system (CNS) gliomas can be classified by characteristic genetic alterations. In addition to solid tissue obtained via surgery or biopsy, cell-free DNA (cfDNA) from cerebrospinal fluid (CSF) is an alternative source of material for genomic analyses.We performed targeted next-generation sequencing (NGS) of CSF cfDNA in a representative cohort of 85 patients presenting at two neurooncological centers with suspicion of primary or recurrent glioma. Copy-number variation (CNV) profiles, single nucleotide variants (SNVs), and small insertions/ deletions (indels) were combined into a molecular-guided tumor classification. Comparison with the solid tumor was performed for 38 cases with matching solid tissue available.Cases were stratified into four groups: glioblastoma (n = 32), other glioma (n = 19), non-malignant (n = 17) and non-diagnostic (n = 17). We introduced a molecular-guided tumor classification, which enabled identification of tumor entities and/ or cancer specific alterations in 75.0 
LB  - PUB:(DE-HGF)16
C6  - pmid:38295147
DO  - DOI:10.1158/1078-0432.CCR-23-2907
UR  - https://inrepo02.dkfz.de/record/287463
ER  -