guest ::
| Home > Publications database > Cerebrospinal fluid cfDNA sequencing for classification of central nervous system glioma. > print |
| Home > Publications database > Cerebrospinal fluid cfDNA sequencing for classification of central nervous system glioma. > print |
| 001 | 287463 | ||
| 005 | 20240715155106.0 | ||
| 024 | 7 | _ | |a 10.1158/1078-0432.CCR-23-2907 |2 doi |
| 024 | 7 | _ | |a pmid:38295147 |2 pmid |
| 024 | 7 | _ | |a 1078-0432 |2 ISSN |
| 024 | 7 | _ | |a 1557-3265 |2 ISSN |
| 024 | 7 | _ | |a altmetric:159009001 |2 altmetric |
| 037 | _ | _ | |a DKFZ-2024-00255 |
| 041 | _ | _ | |a English |
| 082 | _ | _ | |a 610 |
| 100 | 1 | _ | |a Iser, Florian |0 P:(DE-He78)3a5c3ccb1136028578b6bc8fe12b3019 |b 0 |e First author |u dkfz |
| 245 | _ | _ | |a Cerebrospinal fluid cfDNA sequencing for classification of central nervous system glioma. |
| 260 | _ | _ | |a Philadelphia, Pa. [u.a.] |c 2024 |b AACR |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1721051354_17127 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 500 | _ | _ | |a #EA:B320#LA:B320#LA:B300# / 2024 Jul 15;30(14):2974-2985 |
| 520 | _ | _ | |a Primary central nervous system (CNS) gliomas can be classified by characteristic genetic alterations. In addition to solid tissue obtained via surgery or biopsy, cell-free DNA (cfDNA) from cerebrospinal fluid (CSF) is an alternative source of material for genomic analyses.We performed targeted next-generation sequencing (NGS) of CSF cfDNA in a representative cohort of 85 patients presenting at two neurooncological centers with suspicion of primary or recurrent glioma. Copy-number variation (CNV) profiles, single nucleotide variants (SNVs), and small insertions/ deletions (indels) were combined into a molecular-guided tumor classification. Comparison with the solid tumor was performed for 38 cases with matching solid tissue available.Cases were stratified into four groups: glioblastoma (n = 32), other glioma (n = 19), non-malignant (n = 17) and non-diagnostic (n = 17). We introduced a molecular-guided tumor classification, which enabled identification of tumor entities and/ or cancer specific alterations in 75.0 % (n = 24) of glioblastoma and 52.6 % (n = 10) of other glioma cases. The overlap between CSF and matching solid tissue was highest for CNVs (26-48 %) and SNVs at pre-defined gene loci (44 %), followed by SNVs/ indels identified via uninformed variant calling (8-14 %). A molecular-guided tumor classification was possible for 23.5 % (n = 4) of non-diagnostic cases.We developed a targeted sequencing workflow for CSF cfDNA as well as a strategy for interpretation and reporting of sequencing results based on a molecular-guided tumor classification in glioma. |
| 536 | _ | _ | |a 312 - Funktionelle und strukturelle Genomforschung (POF4-312) |0 G:(DE-HGF)POF4-312 |c POF4-312 |f POF IV |x 0 |
| 588 | _ | _ | |a Dataset connected to CrossRef, PubMed, , Journals: inrepo02.dkfz.de |
| 700 | 1 | _ | |a Hinz, Felix |0 P:(DE-He78)8126cfb1cc3fa7fa8fd5c20410c6d863 |b 1 |u dkfz |
| 700 | 1 | _ | |a Hoffmann, Dirk Carsten Frieder |0 P:(DE-He78)e50ff195896914d35f54ed1bf913e270 |b 2 |u dkfz |
| 700 | 1 | _ | |a Grassl, Niklas |0 0000-0001-7987-0444 |b 3 |
| 700 | 1 | _ | |a Güngör, Cansu |0 0009-0002-4405-1427 |b 4 |
| 700 | 1 | _ | |a Meyer, Jochen |0 P:(DE-He78)b6273c0ba3ae37a4d3d1c6b084797f2e |b 5 |u dkfz |
| 700 | 1 | _ | |a Dörner, Laura |0 0009-0001-1528-3889 |b 6 |
| 700 | 1 | _ | |a Hofmann, Lea |0 0009-0001-4651-3659 |b 7 |
| 700 | 1 | _ | |a Kelbch, Vanessa |0 0009-0008-1911-5832 |b 8 |
| 700 | 1 | _ | |a Göbel, Kirsten |0 P:(DE-He78)91f2043e3a8b3c5d31ec010fc634b118 |b 9 |u dkfz |
| 700 | 1 | _ | |a Mahmutoglu, Mustafa Ahmed |b 10 |
| 700 | 1 | _ | |a Vollmuth, Phillipp |0 0000-0002-6224-0064 |b 11 |
| 700 | 1 | _ | |a Patel, Areeba Jamilkhan |0 P:(DE-He78)79506056c05e539af84edf040f0a93ad |b 12 |u dkfz |
| 700 | 1 | _ | |a Nguyen, Duy |0 P:(DE-He78)068f2a9a434bde6bdd0b039f6a7ee5d2 |b 13 |u dkfz |
| 700 | 1 | _ | |a Kaulen, Leon |0 P:(DE-He78)7a5d9437d9f4f1be08abac88e2018fa6 |b 14 |u dkfz |
| 700 | 1 | _ | |a Mildenberger, Iris |0 P:(DE-He78)7daa75b87e6d6e291016ecb54f60b6b3 |b 15 |u dkfz |
| 700 | 1 | _ | |a Sahm, Katharina |0 P:(DE-He78)0f9fbf5fd70dad2bba0760cee65c9613 |b 16 |u dkfz |
| 700 | 1 | _ | |a Maass, Kendra |0 P:(DE-He78)5100059e746b377e2e0a37c0e24f6bf7 |b 17 |u dkfz |
| 700 | 1 | _ | |a Pajtler, Kristian |0 P:(DE-He78)a7c1bbac024fa232d9c6b78443328d9d |b 18 |u dkfz |
| 700 | 1 | _ | |a Shankar, Ganesh M |0 0009-0006-5203-8619 |b 19 |
| 700 | 1 | _ | |a Weiler, Markus |b 20 |
| 700 | 1 | _ | |a Wildemann, Brigitte |0 0000-0002-5389-3338 |b 21 |
| 700 | 1 | _ | |a Winkler, Frank |0 P:(DE-He78)6c294453ee36ad59deddc5494fa6aa4b |b 22 |u dkfz |
| 700 | 1 | _ | |a von Deimling, Andreas |0 P:(DE-He78)a8a10626a848d31e70cfd96a133cc144 |b 23 |u dkfz |
| 700 | 1 | _ | |a Platten, Michael |0 P:(DE-He78)5ef8651b0f857b9c640aa5b1498c43b5 |b 24 |u dkfz |
| 700 | 1 | _ | |a Wick, Wolfgang |0 P:(DE-He78)92e9783ca7025f36ce14e12cd348d2ee |b 25 |u dkfz |
| 700 | 1 | _ | |a Sahm, Felix |0 P:(DE-He78)a1f4b408b9155beb2a8f7cba4d04fe88 |b 26 |e Last author |u dkfz |
| 700 | 1 | _ | |a Kessler, Tobias |0 P:(DE-He78)5c2c9cbe6ce72553684d82d94aebdadd |b 27 |e Last author |u dkfz |
| 773 | _ | _ | |a 10.1158/1078-0432.CCR-23-2907 |0 PERI:(DE-600)2036787-9 |n 14 |p 2974-2985 |t Clinical cancer research |v 30 |y 2024 |x 1078-0432 |
| 909 | C | O | |p VDB |o oai:inrepo02.dkfz.de:287463 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 0 |6 P:(DE-He78)3a5c3ccb1136028578b6bc8fe12b3019 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 1 |6 P:(DE-He78)8126cfb1cc3fa7fa8fd5c20410c6d863 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 2 |6 P:(DE-He78)e50ff195896914d35f54ed1bf913e270 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 3 |6 0000-0001-7987-0444 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 5 |6 P:(DE-He78)b6273c0ba3ae37a4d3d1c6b084797f2e |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 6 |6 0009-0001-1528-3889 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 7 |6 0009-0001-4651-3659 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 8 |6 0009-0008-1911-5832 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 9 |6 P:(DE-He78)91f2043e3a8b3c5d31ec010fc634b118 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 12 |6 P:(DE-He78)79506056c05e539af84edf040f0a93ad |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 13 |6 P:(DE-He78)068f2a9a434bde6bdd0b039f6a7ee5d2 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 14 |6 P:(DE-He78)7a5d9437d9f4f1be08abac88e2018fa6 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 15 |6 P:(DE-He78)7daa75b87e6d6e291016ecb54f60b6b3 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 16 |6 P:(DE-He78)0f9fbf5fd70dad2bba0760cee65c9613 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 17 |6 P:(DE-He78)5100059e746b377e2e0a37c0e24f6bf7 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 18 |6 P:(DE-He78)a7c1bbac024fa232d9c6b78443328d9d |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 22 |6 P:(DE-He78)6c294453ee36ad59deddc5494fa6aa4b |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 23 |6 P:(DE-He78)a8a10626a848d31e70cfd96a133cc144 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 24 |6 P:(DE-He78)5ef8651b0f857b9c640aa5b1498c43b5 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 25 |6 P:(DE-He78)92e9783ca7025f36ce14e12cd348d2ee |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 26 |6 P:(DE-He78)a1f4b408b9155beb2a8f7cba4d04fe88 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 27 |6 P:(DE-He78)5c2c9cbe6ce72553684d82d94aebdadd |
| 913 | 1 | _ | |a DE-HGF |b Gesundheit |l Krebsforschung |1 G:(DE-HGF)POF4-310 |0 G:(DE-HGF)POF4-312 |3 G:(DE-HGF)POF4 |2 G:(DE-HGF)POF4-300 |4 G:(DE-HGF)POF |v Funktionelle und strukturelle Genomforschung |x 0 |
| 914 | 1 | _ | |y 2024 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0300 |2 StatID |b Medline |d 2023-08-25 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0199 |2 StatID |b Clarivate Analytics Master Journal List |d 2023-08-25 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1050 |2 StatID |b BIOSIS Previews |d 2023-08-25 |
| 915 | _ | _ | |a WoS |0 StatID:(DE-HGF)0113 |2 StatID |b Science Citation Index Expanded |d 2023-08-25 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0150 |2 StatID |b Web of Science Core Collection |d 2023-08-25 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1190 |2 StatID |b Biological Abstracts |d 2023-08-25 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0160 |2 StatID |b Essential Science Indicators |d 2023-08-25 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1110 |2 StatID |b Current Contents - Clinical Medicine |d 2023-08-25 |
| 915 | _ | _ | |a JCR |0 StatID:(DE-HGF)0100 |2 StatID |b CLIN CANCER RES : 2022 |d 2023-08-25 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0200 |2 StatID |b SCOPUS |d 2023-08-25 |
| 915 | _ | _ | |a IF >= 10 |0 StatID:(DE-HGF)9910 |2 StatID |b CLIN CANCER RES : 2022 |d 2023-08-25 |
| 920 | 2 | _ | |0 I:(DE-He78)B320-20160331 |k B320 |l KKE Neuroonkologie |x 0 |
| 920 | 2 | _ | |0 I:(DE-He78)B300-20160331 |k B300 |l KKE Neuropathologie |x 1 |
| 920 | 1 | _ | |0 I:(DE-He78)B320-20160331 |k B320 |l KKE Neuroonkologie |x 0 |
| 920 | 1 | _ | |0 I:(DE-He78)HD01-20160331 |k HD01 |l DKTK HD zentral |x 1 |
| 920 | 1 | _ | |0 I:(DE-He78)B300-20160331 |k B300 |l KKE Neuropathologie |x 2 |
| 920 | 1 | _ | |0 I:(DE-He78)D170-20160331 |k D170 |l KKE Neuroimmunologie und Hirntumorimmunologie |x 3 |
| 920 | 1 | _ | |0 I:(DE-He78)W610-20160331 |k W610 |l Core Facility Omics IT |x 4 |
| 920 | 1 | _ | |0 I:(DE-He78)B062-20160331 |k B062 |l B062 Pädiatrische Neuroonkologie |x 5 |
| 920 | 0 | _ | |0 I:(DE-He78)B320-20160331 |k B320 |l KKE Neuroonkologie |x 0 |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a I:(DE-He78)B320-20160331 |
| 980 | _ | _ | |a I:(DE-He78)HD01-20160331 |
| 980 | _ | _ | |a I:(DE-He78)B300-20160331 |
| 980 | _ | _ | |a I:(DE-He78)D170-20160331 |
| 980 | _ | _ | |a I:(DE-He78)W610-20160331 |
| 980 | _ | _ | |a I:(DE-He78)B062-20160331 |
| 980 | _ | _ | |a UNRESTRICTED |
| Library | Collection | CLSMajor | CLSMinor | Language | Author |
|---|