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@ARTICLE{Panten:288057,
author = {J. Panten$^*$ and T. Heinen$^*$ and C. Ernst and N. Eling
and R. E. Wagner$^*$ and M. Satorius$^*$ and J. C. Marioni
and O. Stegle$^*$ and D. Odom$^*$},
title = {{T}he dynamic genetic determinants of increased
transcriptional divergence in spermatids.},
journal = {Nature Communications},
volume = {15},
number = {1},
issn = {2041-1723},
address = {[London]},
publisher = {Nature Publishing Group UK},
reportid = {DKFZ-2024-00315},
pages = {1272},
year = {2024},
note = {#EA:B260#EA:B270#LA:B260#LA:B270#},
abstract = {Cis-genetic effects are key determinants of transcriptional
divergence in discrete tissues and cell types. However, how
cis- and trans-effects act across continuous trajectories of
cellular differentiation in vivo is poorly understood. Here,
we quantify allele-specific expression during spermatogenic
differentiation at single-cell resolution in an F1 hybrid
mouse system, allowing for the comprehensive
characterisation of cis- and trans-genetic effects,
including their dynamics across cellular differentiation.
Collectively, almost half of the genes subject to genetic
regulation show evidence for dynamic cis-effects that vary
during differentiation. Our system also allows us to
robustly identify dynamic trans-effects, which are less
pervasive than cis-effects. In aggregate, genetic effects
were strongest in round spermatids, which parallels their
increased transcriptional divergence we identified between
species. Our approach provides a comprehensive
quantification of the variability of genetic effects in
vivo, and demonstrates a widely applicable strategy to
dissect the impact of regulatory variants on gene regulation
in dynamic systems.},
cin = {B270 / B260 / A350},
ddc = {500},
cid = {I:(DE-He78)B270-20160331 / I:(DE-He78)B260-20160331 /
I:(DE-He78)A350-20160331},
pnm = {312 - Funktionelle und strukturelle Genomforschung
(POF4-312)},
pid = {G:(DE-HGF)POF4-312},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:38341412},
pmc = {pmc:PMC10858866},
doi = {10.1038/s41467-024-45133-1},
url = {https://inrepo02.dkfz.de/record/288057},
}