%0 Journal Article
%A Jeske, Rima
%A Becker, Nico
%A Kroeller, Lea
%A Mentzer, Alexander J
%A Brenner, Nicole
%A Guy, Edward
%A Waterboer, Tim
%A Butt, Julia Anna
%T Advancing Toxoplasma gondii multiplex serology.
%J Microbiology spectrum
%V 12
%N 4
%@ 2165-0497
%C Birmingham, Ala.
%I ASM
%M DKFZ-2024-00406
%P e0361823
%D 2024
%Z #EA:F020#LA:F020# / 2024 Apr 2;12(4):e0361823 / NEU/ #EA:D320#LA:D320#
%X Toxoplasma gondii is a highly prevalent pathogen causing zoonotic infections with significant public health implications. Yet, our understanding of long-term consequences, associated risk factors, and the potential role of co-infections is still limited. Seroepidemiological studies are a valuable approach to address open questions and enhance our insights into T. gondii across human populations. Here, we present substantial advancements to our previously developed T. gondii multiplex serology assay, which is based on the immunodominant antigens SAG1 and P22. While our previous bead-based assay quantified antibody levels against multiple targets in a high-throughput fashion requiring only a small sample volume, impaired assay characteristics emerged in sample dilutions beyond 1:100 and when being transferred to magnetic beads. Both are now critical for inclusion in large-scale seroprevalence studies. Using the truncated versions, SAG1D1 and P22trunc, significantly enhanced signal-to-noise ratios were achieved with almost perfect concordance with the gold-standard Sabin-Feldman dye test. In sample dilutions of 1:100, the diagnostic accuracy of SAG1D1 and P22trunc reached sensitivities (true positive rates) of 98
%K Toxoplasma gondii (Other)
%K high-throughput serology (Other)
%K multiplex serology (Other)
%K public health (Other)
%K seroepidemiology (Other)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:38385741
%R 10.1128/spectrum.03618-23
%U https://inrepo02.dkfz.de/record/288542