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@ARTICLE{Bundschuh:288580,
author = {C. Bundschuh and N. Weidner and J. Klein and T. Rausch and
N. Azevedo and A. Telzerow and J.-P. Mallm$^*$ and H. Kim
and S. Steiger$^*$ and I. Seufert$^*$ and K. Börner and K.
Bauer$^*$ and D. Hübschmann$^*$ and K. L. Jost and S.
Parthé and P. Schnitzler and M. Boutros$^*$ and K.
Rippe$^*$ and B. Müller and R. Bartenschlager$^*$ and H.-G.
Kräusslich and V. Benes},
title = {{E}volution of {SARS}-{C}o{V}-2 in the
{R}hine-{N}eckar/{H}eidelberg {R}egion 01/2021 - 07/2023.},
journal = {Infection, genetics and evolution},
volume = {119},
issn = {1567-1348},
address = {Amsterdam [u.a.]},
publisher = {Elsevier Science},
reportid = {DKFZ-2024-00426},
pages = {105577},
year = {2024},
note = {2024 Apr;119:105577},
abstract = {In January 2021, the monitoring of circulating variants of
SARS-CoV-2 was initiated in Germany under the Corona
Surveillance Act, which was discontinued after July 2023.
This initiative aimed to enhance pandemic containment, as
specific amino acid changes, particularly in the spike
protein, were associated with increased transmission and
reduced vaccine efficacy. Our group conducted whole genome
sequencing using the ARTIC protocol (currently V4) on
Illumina's NextSeq 500 platform (and, starting in May 2023,
on the MiSeq DX platform) for SARS-CoV-2 positive specimen
from patients at Heidelberg University Hospital, associated
hospitals, and the public health office in the
Rhine-Neckar/Heidelberg region. In total, we sequenced
26,795 SARS-CoV-2-positive samples between January 2021 and
July 2023. Valid sequences, meeting the requirements for
upload to the German electronic sequencing data hub (DESH)
operated by the Robert Koch Institute (RKI), were determined
for 24,852 samples, and the lineage/clade could be
identified for 25,912 samples. The year 2021 witnessed
significant dynamics in the circulating variants in the
Rhine-Neckar/Heidelberg region, including A.27.RN, followed
by the emergence of B.1.1.7 (Alpha), subsequently displaced
by B.1.617.2 (Delta), and the initial occurrences of
B.1.1.529 (Omicron). By January 2022, B.1.1.529 had
superseded B.1.617.2, dominating with over $90\%.$ The years
2022 and 2023 were then characterized by the dominance of
B.1.1.529 and its sublineages, particularly BA.5 and BA.2,
and more recently, the emergence of recombinant variants
like XBB.1.5. Since the global dominance of B.1.617.2, the
identified variant distribution in our local study, apart
from a time delay in the spread of new variants, can be
considered largely representative of the global
distribution. om a time delay in the spread of new variants,
can be considered largely representative of the global
distribution.},
keywords = {ARTIC protocol (Other) / Bioinformatics (Other) /
Heidelberg/Rhine Neckar region (Other) / SARS-CoV-2 (Other)
/ Whole genome sequencing (Other)},
cin = {W192 / B066 / A010 / B110 / F170 / D430},
ddc = {570},
cid = {I:(DE-He78)W192-20160331 / I:(DE-He78)B066-20160331 /
I:(DE-He78)A010-20160331 / I:(DE-He78)B110-20160331 /
I:(DE-He78)F170-20160331 / I:(DE-He78)D430-20160331},
pnm = {314 - Immunologie und Krebs (POF4-314)},
pid = {G:(DE-HGF)POF4-314},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:38403035},
doi = {10.1016/j.meegid.2024.105577},
url = {https://inrepo02.dkfz.de/record/288580},
}