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@ARTICLE{GalveiasCasquinhaPiresMartins:288879,
author = {L. Galveias Casquinha Pires Martins$^*$ and L.
Sieverling$^*$ and M. Michelhans$^*$ and C. Schiller$^*$ and
C. Erkut$^*$ and T. Grünewald$^*$ and S. Triana and S.
Fröhling$^*$ and L. Velten and H. Glimm$^*$ and C.
Scholl$^*$},
title = {{S}ingle-cell division tracing and transcriptomics reveal
cell types and differentiation paths in the regenerating
lung.},
journal = {Nature Communications},
volume = {15},
number = {1},
issn = {2041-1723},
address = {[London]},
publisher = {Nature Publishing Group UK},
reportid = {DKFZ-2024-00521},
pages = {2246},
year = {2024},
note = {#LA:B290#LA:B290#},
abstract = {Understanding the molecular and cellular processes involved
in lung epithelial regeneration may fuel the development of
therapeutic approaches for lung diseases. We combine mouse
models allowing diphtheria toxin-mediated damage of specific
epithelial cell types and parallel GFP-labeling of
functionally dividing cells with single-cell transcriptomics
to characterize the regeneration of the distal lung. We
uncover cell types, including Krt13+ basal and Krt15+ club
cells, detect an intermediate cell state between basal and
goblet cells, reveal goblet cells as actively dividing
progenitor cells, and provide evidence that adventitial
fibroblasts act as supporting cells in epithelial
regeneration. We also show that diphtheria toxin-expressing
cells can persist in the lung, express specific inflammatory
factors, and transcriptionally resemble a previously
undescribed population in the lungs of COVID-19 patients.
Our study provides a comprehensive single-cell atlas of the
distal lung that characterizes early transcriptional and
cellular responses to concise epithelial injury,
encompassing proliferation, differentiation, and
cell-to-cell interactions.},
cin = {B290 / B340 / B410 / HD01 / DD01 / B280},
ddc = {500},
cid = {I:(DE-He78)B290-20160331 / I:(DE-He78)B340-20160331 /
I:(DE-He78)B410-20160331 / I:(DE-He78)HD01-20160331 /
I:(DE-He78)DD01-20160331 / I:(DE-He78)B280-20160331},
pnm = {312 - Funktionelle und strukturelle Genomforschung
(POF4-312)},
pid = {G:(DE-HGF)POF4-312},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:38472236},
doi = {10.1038/s41467-024-46469-4},
url = {https://inrepo02.dkfz.de/record/288879},
}