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000288978 1001_ $$0P:(DE-He78)4569ef2919d2438765ad71515f53646b$$aGómez-Zepeda, David$$b0$$eFirst author$$udkfz
000288978 245__ $$aThunder-DDA-PASEF enables high-coverage immunopeptidomics and is boosted by MS2Rescore with MS2PIP timsTOF fragmentation prediction model.
000288978 260__ $$a[London]$$bNature Publishing Group UK$$c2024
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000288978 520__ $$aHuman leukocyte antigen (HLA) class I peptide ligands (HLAIps) are key targets for developing vaccines and immunotherapies against infectious pathogens or cancer cells. Identifying HLAIps is challenging due to their high diversity, low abundance, and patient individuality. Here, we develop a highly sensitive method for identifying HLAIps using liquid chromatography-ion mobility-tandem mass spectrometry (LC-IMS-MS/MS). In addition, we train a timsTOF-specific peak intensity MS2PIP model for tryptic and non-tryptic peptides and implement it in MS2Rescore (v3) together with the CCS predictor from ionmob. The optimized method, Thunder-DDA-PASEF, semi-selectively fragments singly and multiply charged HLAIps based on their IMS and m/z. Moreover, the method employs the high sensitivity mode and extended IMS resolution with fewer MS/MS frames (300 ms TIMS ramp, 3 MS/MS frames), doubling the coverage of immunopeptidomics analyses, compared to the proteomics-tailored DDA-PASEF (100 ms TIMS ramp, 10 MS/MS frames). Additionally, rescoring boosts the HLAIps identification by 41.7% to 33%, resulting in 5738 HLAIps from as little as one million JY cell equivalents, and 14,516 HLAIps from 20 million. This enables in-depth profiling of HLAIps from diverse human cell lines and human plasma. Finally, profiling JY and Raji cells transfected to express the SARS-CoV-2 spike protein results in 16 spike HLAIps, thirteen of which have been reported to elicit immune responses in human patients.
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000288978 7001_ $$aArnold-Schild, Danielle$$b1
000288978 7001_ $$0P:(DE-He78)2678e81a8716fea510f7fc0d2b06bb27$$aBeyrle, Julian$$b2$$udkfz
000288978 7001_ $$aDeclercq, Arthur$$b3
000288978 7001_ $$00000-0002-1679-1711$$aGabriels, Ralf$$b4
000288978 7001_ $$aKumm, Elena$$b5
000288978 7001_ $$aPreikschat, Annica$$b6
000288978 7001_ $$aŁącki, Mateusz Krzysztof$$b7
000288978 7001_ $$00000-0001-5066-6263$$aHirschler, Aurélie$$b8
000288978 7001_ $$00009-0009-6839-9947$$aRijal, Jeewan Babu$$b9
000288978 7001_ $$00000-0002-0079-319X$$aCarapito, Christine$$b10
000288978 7001_ $$00000-0003-4277-658X$$aMartens, Lennart$$b11
000288978 7001_ $$00000-0002-8031-6384$$aDistler, Ute$$b12
000288978 7001_ $$aSchild, Hansjörg$$b13
000288978 7001_ $$0P:(DE-He78)74e391c68d7926be83d679f3d8891e33$$aTenzer, Stefan$$b14$$eLast author$$udkfz
000288978 773__ $$0PERI:(DE-600)2553671-0$$a10.1038/s41467-024-46380-y$$gVol. 15, no. 1, p. 2288$$n1$$p2288$$tNature Communications$$v15$$x2041-1723$$y2024
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