Home > Publications database > APOBEC2 safeguards skeletal muscle cell fate through binding chromatin and regulating transcription of non-muscle genes during myoblast differentiation. > print

001     289494
005     20241114182511.0
024 7 _ |a 10.1073/pnas.2312330121
|2 doi
024 7 _ |a pmid:38625936
|2 pmid
024 7 _ |a 0027-8424
|2 ISSN
024 7 _ |a 1091-6490
|2 ISSN
024 7 _ |a altmetric:162564945
|2 altmetric
037 _ _ |a DKFZ-2024-00816
041 _ _ |a English
082 _ _ |a 500
100 1 _ |a Lorenzo, Jose Paulo
|0 P:(DE-He78)2514ea6c79ba09982b07649cbda6645b
|b 0
|e First author
|u dkfz
245 _ _ |a APOBEC2 safeguards skeletal muscle cell fate through binding chromatin and regulating transcription of non-muscle genes during myoblast differentiation.
260 _ _ |a Washington, DC
|c 2024
|b National Acad. of Sciences
336 7 _ |a article
|2 DRIVER
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|b journal
|m journal
|0 PUB:(DE-HGF)16
|s 1713444211_13215
|2 PUB:(DE-HGF)
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a Journal Article
|0 0
|2 EndNote
500 _ _ |a #EA:D150#LA:D150#
520 _ _ |a The apolipoprotein B messenger RNA editing enzyme, catalytic polypeptide (APOBEC) family is composed of nucleic acid editors with roles ranging from antibody diversification to RNA editing. APOBEC2, a member of this family with an evolutionarily conserved nucleic acid-binding cytidine deaminase domain, has neither an established substrate nor function. Using a cellular model of muscle differentiation where APOBEC2 is inducibly expressed, we confirmed that APOBEC2 does not have the attributed molecular functions of the APOBEC family, such as RNA editing, DNA demethylation, and DNA mutation. Instead, we found that during muscle differentiation APOBEC2 occupied a specific motif within promoter regions; its removal from those regions resulted in transcriptional changes. Mechanistically, these changes reflect the direct interaction of APOBEC2 with histone deacetylase (HDAC) transcriptional corepressor complexes. We also found that APOBEC2 could bind DNA directly, in a sequence-specific fashion, suggesting that it functions as a recruiter of HDAC to specific genes whose promoters it occupies. These genes are normally suppressed during muscle cell differentiation, and their suppression may contribute to the safeguarding of muscle cell fate. Altogether, our results reveal a unique role for APOBEC2 within the APOBEC family.
536 _ _ |a 314 - Immunologie und Krebs (POF4-314)
|0 G:(DE-HGF)POF4-314
|c POF4-314
|f POF IV
|x 0
588 _ _ |a Dataset connected to CrossRef, PubMed, , Journals: inrepo02.dkfz.de
650 _ 7 |a APOBEC family
|2 Other
650 _ 7 |a DNA binding
|2 Other
650 _ 7 |a muscle differentiation
|2 Other
650 _ 7 |a safeguard factor
|2 Other
650 _ 7 |a transcriptional regulator
|2 Other
650 _ 7 |a Chromatin
|2 NLM Chemicals
650 _ 7 |a Muscle Proteins
|2 NLM Chemicals
650 _ 7 |a APOBEC Deaminases
|0 EC 3.5.4.5
|2 NLM Chemicals
650 _ 7 |a Cytidine Deaminase
|0 EC 3.5.4.5
|2 NLM Chemicals
650 _ 7 |a RNA, Messenger
|2 NLM Chemicals
650 _ 7 |a DNA
|0 9007-49-2
|2 NLM Chemicals
650 _ 7 |a APOBEC-1 Deaminase
|0 EC 3.5.4.36
|2 NLM Chemicals
650 _ 2 |a Chromatin: genetics
|2 MeSH
650 _ 2 |a Muscle Proteins: metabolism
|2 MeSH
650 _ 2 |a APOBEC Deaminases: genetics
|2 MeSH
650 _ 2 |a Cytidine Deaminase: metabolism
|2 MeSH
650 _ 2 |a Muscle Fibers, Skeletal: metabolism
|2 MeSH
650 _ 2 |a Cell Differentiation: genetics
|2 MeSH
650 _ 2 |a RNA, Messenger: genetics
|2 MeSH
650 _ 2 |a Myoblasts: metabolism
|2 MeSH
650 _ 2 |a DNA
|2 MeSH
650 _ 2 |a APOBEC-1 Deaminase: genetics
|2 MeSH
700 1 _ |a Molla, Linda
|b 1
700 1 _ |a Amro, Elias M. E.
|0 P:(DE-He78)1bbd2b961460d6dad83b63ac2004fd3b
|b 2
|e First author
|u dkfz
700 1 _ |a Ibarra, Ignacio L
|b 3
700 1 _ |a Ruf, Sandra
|0 P:(DE-He78)987a16a93296d4a043a1b2337d224b53
|b 4
|u dkfz
700 1 _ |a Neber, Cedrik
|0 P:(DE-He78)2a4b1dc59dce9461adf2a4e237efef7c
|b 5
|u dkfz
700 1 _ |a Gkougkousis, Christos
|0 0009-0009-1640-3457
|b 6
700 1 _ |a Ridani, Jana
|b 7
700 1 _ |a Subramani, Poorani Ganesh
|b 8
700 1 _ |a Boulais, Jonathan
|b 9
700 1 _ |a Harjanto, Dewi
|b 10
700 1 _ |a Vonica, Alin
|0 0000-0001-8044-9569
|b 11
700 1 _ |a Di Noia, Javier M
|0 0000-0003-2896-0321
|b 12
700 1 _ |a Dieterich, Christoph
|0 0000-0001-9468-6311
|b 13
700 1 _ |a Zaugg, Judith B
|b 14
700 1 _ |a Papavasiliou, Fotini
|0 P:(DE-He78)79dbda58d51b613a0a421f579d2dfed3
|b 15
|e Last author
|u dkfz
773 _ _ |a 10.1073/pnas.2312330121
|g Vol. 121, no. 17, p. e2312330121
|0 PERI:(DE-600)1461794-8
|n 17
|p e2312330121
|t Proceedings of the National Academy of Sciences of the United States of America
|v 121
|y 2024
|x 0027-8424
909 C O |o oai:inrepo02.dkfz.de:289494
|p VDB
|p OpenAPC
|p openCost
910 1 _ |a Deutsches Krebsforschungszentrum
|0 I:(DE-588b)2036810-0
|k DKFZ
|b 0
|6 P:(DE-He78)2514ea6c79ba09982b07649cbda6645b
910 1 _ |a Deutsches Krebsforschungszentrum
|0 I:(DE-588b)2036810-0
|k DKFZ
|b 2
|6 P:(DE-He78)1bbd2b961460d6dad83b63ac2004fd3b
910 1 _ |a Deutsches Krebsforschungszentrum
|0 I:(DE-588b)2036810-0
|k DKFZ
|b 4
|6 P:(DE-He78)987a16a93296d4a043a1b2337d224b53
910 1 _ |a Deutsches Krebsforschungszentrum
|0 I:(DE-588b)2036810-0
|k DKFZ
|b 5
|6 P:(DE-He78)2a4b1dc59dce9461adf2a4e237efef7c
910 1 _ |a Deutsches Krebsforschungszentrum
|0 I:(DE-588b)2036810-0
|k DKFZ
|b 15
|6 P:(DE-He78)79dbda58d51b613a0a421f579d2dfed3
913 1 _ |a DE-HGF
|b Gesundheit
|l Krebsforschung
|1 G:(DE-HGF)POF4-310
|0 G:(DE-HGF)POF4-314
|3 G:(DE-HGF)POF4
|2 G:(DE-HGF)POF4-300
|4 G:(DE-HGF)POF
|v Immunologie und Krebs
|x 0
914 1 _ |y 2024
915 _ _ |a National-Konsortium
|0 StatID:(DE-HGF)0430
|2 StatID
|d 2023-08-26
|w ger
915 _ _ |a JCR
|0 StatID:(DE-HGF)0100
|2 StatID
|b P NATL ACAD SCI USA : 2022
|d 2023-08-26
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0200
|2 StatID
|b SCOPUS
|d 2023-08-26
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0300
|2 StatID
|b Medline
|d 2023-08-26
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0320
|2 StatID
|b PubMed Central
|d 2023-08-26
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0600
|2 StatID
|b Ebsco Academic Search
|d 2023-08-26
915 _ _ |a Peer Review
|0 StatID:(DE-HGF)0030
|2 StatID
|b ASC
|d 2023-08-26
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0199
|2 StatID
|b Clarivate Analytics Master Journal List
|d 2023-08-26
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1050
|2 StatID
|b BIOSIS Previews
|d 2023-08-26
915 _ _ |a WoS
|0 StatID:(DE-HGF)0113
|2 StatID
|b Science Citation Index Expanded
|d 2023-08-26
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0150
|2 StatID
|b Web of Science Core Collection
|d 2023-08-26
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1030
|2 StatID
|b Current Contents - Life Sciences
|d 2023-08-26
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1190
|2 StatID
|b Biological Abstracts
|d 2023-08-26
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1060
|2 StatID
|b Current Contents - Agriculture, Biology and Environmental Sciences
|d 2023-08-26
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1040
|2 StatID
|b Zoological Record
|d 2023-08-26
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0160
|2 StatID
|b Essential Science Indicators
|d 2023-08-26
915 _ _ |a IF >= 10
|0 StatID:(DE-HGF)9910
|2 StatID
|b P NATL ACAD SCI USA : 2022
|d 2023-08-26
915 p c |a APC keys set
|2 APC
|0 PC:(DE-HGF)0000
915 p c |a Local Funding
|2 APC
|0 PC:(DE-HGF)0001
920 2 _ |0 I:(DE-He78)D150-20160331
|k D150
|l D150 Immundiversität
|x 0
920 1 _ |0 I:(DE-He78)D150-20160331
|k D150
|l D150 Immundiversität
|x 0
920 0 _ |0 I:(DE-He78)D150-20160331
|k D150
|l D150 Immundiversität
|x 0
980 _ _ |a journal
980 _ _ |a VDB
980 _ _ |a I:(DE-He78)D150-20160331
980 _ _ |a UNRESTRICTED
980 _ _ |a APC

LibraryCollectionCLSMajorCLSMinorLanguageAuthor
Marc 21