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@ARTICLE{Stranak:289899,
      author       = {Z. Stranak and T. Ardan and Y. Nemesh and M. Toms and L.
                      Toualbi and R. Harbottle$^*$ and Z. Ellederova and L.
                      Lytvynchuk and G. Petrovski and J. Motlik and M. Moosajee
                      and I. Kozak},
      title        = {{F}easibility of {D}irect {V}itrectomy-{S}paring
                      {S}ubretinal {I}njection for {G}ene {D}elivery in {L}arge
                      {A}nimals.},
      journal      = {Current eye research},
      volume       = {49},
      number       = {8},
      issn         = {0271-3683},
      address      = {Abingdon},
      publisher    = {Taylor $\&$ Francis Group},
      reportid     = {DKFZ-2024-00891},
      pages        = {879-887},
      year         = {2024},
      note         = {2024 Aug;49(8):879-887},
      abstract     = {To assess the safety and feasibility of direct
                      vitrectomy-sparing subretinal injection for gene delivery in
                      a large animal model.The experimental Liběchov minipigs
                      were used for subretinal delivery of a plasmid DNA vector
                      (pS/MAR-CMV-copGFP) with cytomegalovirus (CMV) promoter,
                      green fluorescent protein (GFP) reporter (copGFP) and a
                      scaffold/matrix attachment region (S/MAR) sequence. The eyes
                      were randomized to subretinal injection of the vector
                      following pars plana vitrectomy (control group) or a direct
                      injection without prior vitrectomy surgery (experimental
                      group). Intra- and post-operative observations up to 30 days
                      after surgery were compared.Six eyes of three mini-pigs
                      underwent surgery for delivery into the subretinal space.
                      Two eyes in the control group were operated with a classical
                      approach (lens-sparing vitrectomy and posterior hyaloid
                      detachment). The other four eyes in the experimental group
                      were injected directly with a subretinal cannula without
                      vitrectomy surgery. No adverse events, such as
                      endophthalmitis, retinal detachment and intraocular pressure
                      elevation were observed post-operatively. The eyes in the
                      experimental group had both shorter surgical time and
                      recovery while achieving the same surgical goal.This pilot
                      study demonstrates that successful subretinal delivery of
                      gene therapy vectors is achievable using a direct injection
                      without prior vitrectomy surgery.},
      keywords     = {Subretinal injection (Other) / large animal (Other) /
                      non-viral gene vector (Other) / pars plana vitrectomy
                      (Other) / safety (Other)},
      cin          = {D420 / F160},
      ddc          = {610},
      cid          = {I:(DE-He78)D420-20160331 / I:(DE-He78)F160-20160331},
      pnm          = {314 - Immunologie und Krebs (POF4-314)},
      pid          = {G:(DE-HGF)POF4-314},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:38666493},
      doi          = {10.1080/02713683.2024.2343335},
      url          = {https://inrepo02.dkfz.de/record/289899},
}