TY  - JOUR
AU  - Corazzi, Lorenzo
AU  - Ionasz, Vivien
AU  - Andrejev, Sergej
AU  - Wang, Li-Chin
AU  - Vouzas, Athanasios
AU  - Giaisi, Marco
AU  - Di Muzio, Giulia
AU  - Ding, Boyu
AU  - Marx, Anna
AU  - Henkenjohann, Jonas
AU  - Allers, Michael
AU  - Gilbert, David M
AU  - Wei, Pei-Chi
TI  - Linear interaction between replication and transcription shapes DNA break dynamics at recurrent DNA break Clusters.
JO  - Nature Communications
VL  - 15
IS  - 1
SN  - 2041-1723
CY  - [London]
PB  - Nature Publishing Group UK
M1  - DKFZ-2024-00895
SP  - 3594
PY  - 2024
AB  - Recurrent DNA break clusters (RDCs) are replication-transcription collision hotspots; many are unique to neural progenitor cells. Through high-resolution replication sequencing and a capture-ligation assay in mouse neural progenitor cells experiencing replication stress, we unravel the replication features dictating RDC location and orientation. Most RDCs occur at the replication forks traversing timing transition regions (TTRs), where sparse replication origins connect unidirectional forks. Leftward-moving forks generate telomere-connected DNA double-strand breaks (DSBs), while rightward-moving forks lead to centromere-connected DSBs. Strand-specific mapping for DNA-bound RNA reveals co-transcriptional dual-strand DNA:RNA hybrids present at a higher density in RDC than in other actively transcribed long genes. In addition, mapping RNA polymerase activity uncovers that head-to-head interactions between replication and transcription machinery result in 60
KW  - Animals
KW  - Transcription, Genetic
KW  - DNA Breaks, Double-Stranded
KW  - Mice
KW  - DNA Replication
KW  - Genomic Instability
KW  - Neural Stem Cells: metabolism
KW  - DNA: metabolism
KW  - DNA: genetics
KW  - Replication Origin
KW  - Telomere: metabolism
KW  - Telomere: genetics
KW  - Centromere: metabolism
KW  - Centromere: genetics
KW  - DNA (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:38678011
DO  - DOI:10.1038/s41467-024-47934-w
UR  - https://inrepo02.dkfz.de/record/289903
ER  -