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@ARTICLE{Corazzi:289903,
      author       = {L. Corazzi$^*$ and V. Ionasz$^*$ and S. Andrejev$^*$ and
                      L.-C. Wang$^*$ and A. Vouzas and M. Giaisi$^*$ and G. Di
                      Muzio$^*$ and B. Ding$^*$ and A. Marx$^*$ and J.
                      Henkenjohann$^*$ and M. Allers$^*$ and D. M. Gilbert and
                      P.-C. Wei$^*$},
      title        = {{L}inear interaction between replication and transcription
                      shapes {DNA} break dynamics at recurrent {DNA} break
                      {C}lusters.},
      journal      = {Nature Communications},
      volume       = {15},
      number       = {1},
      issn         = {2041-1723},
      address      = {[London]},
      publisher    = {Nature Publishing Group UK},
      reportid     = {DKFZ-2024-00895},
      pages        = {3594},
      year         = {2024},
      abstract     = {Recurrent DNA break clusters (RDCs) are
                      replication-transcription collision hotspots; many are
                      unique to neural progenitor cells. Through high-resolution
                      replication sequencing and a capture-ligation assay in mouse
                      neural progenitor cells experiencing replication stress, we
                      unravel the replication features dictating RDC location and
                      orientation. Most RDCs occur at the replication forks
                      traversing timing transition regions (TTRs), where sparse
                      replication origins connect unidirectional forks.
                      Leftward-moving forks generate telomere-connected DNA
                      double-strand breaks (DSBs), while rightward-moving forks
                      lead to centromere-connected DSBs. Strand-specific mapping
                      for DNA-bound RNA reveals co-transcriptional dual-strand
                      DNA:RNA hybrids present at a higher density in RDC than in
                      other actively transcribed long genes. In addition, mapping
                      RNA polymerase activity uncovers that head-to-head
                      interactions between replication and transcription machinery
                      result in $60\%$ DSB contribution to the head-on compared to
                      $40\%$ for co-directional. Taken together we reveal TTR as a
                      fragile class and show how the linear interaction between
                      transcription and replication impacts genome stability.},
      keywords     = {Animals / Transcription, Genetic / DNA Breaks,
                      Double-Stranded / Mice / DNA Replication / Genomic
                      Instability / Neural Stem Cells: metabolism / DNA:
                      metabolism / DNA: genetics / Replication Origin / Telomere:
                      metabolism / Telomere: genetics / Centromere: metabolism /
                      Centromere: genetics / DNA (NLM Chemicals)},
      cin          = {B400},
      ddc          = {500},
      cid          = {I:(DE-He78)B400-20160331},
      pnm          = {312 - Funktionelle und strukturelle Genomforschung
                      (POF4-312)},
      pid          = {G:(DE-HGF)POF4-312},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:38678011},
      doi          = {10.1038/s41467-024-47934-w},
      url          = {https://inrepo02.dkfz.de/record/289903},
}