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@ARTICLE{Obacz:290039,
author = {J. Obacz and J. Archambeau and E. Lafont and M. Nivet and
S. Martin and M. Aubry and K. Voutetakis and R. Pineau and
R. Boniface and D. Sicari and D. Pelizzari-Raymundo and G.
Ghukasyan and E. McGrath and E.-I. Vlachavas$^*$ and M. Le
Gallo and P. J. Le Reste and K. Barroso and T. Fainsod-Levi
and A. Obiedat and Z. Granot and B. Tirosh and J. Samal and
A. Pandit and L. Négroni and N. Soriano and A. Monnier and
J. Mosser and A. Chatziioannou$^*$ and V. Quillien and E.
Chevet and T. Avril},
title = {{IRE}1 endoribonuclease signaling promotes myeloid cell
infiltration in glioblastoma.},
journal = {Neuro-Oncology},
volume = {26},
number = {5},
issn = {1522-8517},
address = {Oxford},
publisher = {Oxford Univ. Press},
reportid = {DKFZ-2024-00944},
pages = {858 - 871},
year = {2024},
abstract = {Intrinsic or environmental stresses trigger the
accumulation of improperly folded proteins in the
endoplasmic reticulum (ER), leading to ER stress. To cope
with this, cells have evolved an adaptive mechanism named
the unfolded protein response (UPR) which is hijacked by
tumor cells to develop malignant features. Glioblastoma
(GB), the most aggressive and lethal primary brain tumor,
relies on UPR to sustain growth. We recently showed that
IRE1 alpha (referred to IRE1 hereafter), 1 of the UPR
transducers, promotes GB invasion, angiogenesis, and
infiltration by macrophage. Hence, high tumor IRE1 activity
in tumor cells predicts a worse outcome. Herein, we
characterized the IRE1-dependent signaling that shapes the
immune microenvironment toward monocytes/macrophages and
neutrophils.We used human and mouse cellular models in which
IRE1 was genetically or pharmacologically invalidated and
which were tested in vivo. Publicly available datasets from
GB patients were also analyzed to confirm our findings.We
showed that IRE1 signaling, through both the transcription
factor XBP1s and the regulated IRE1-dependent decay controls
the expression of the ubiquitin-conjugating E2 enzyme
UBE2D3. In turn, UBE2D3 activates the NFκB pathway,
resulting in chemokine production and myeloid infiltration
in tumors.Our work identifies a novel IRE1/UBE2D3
proinflammatory axis that plays an instrumental role in GB
immune regulation.},
keywords = {Glioblastoma: pathology / Glioblastoma: metabolism / Humans
/ Mice / Endoribonucleases: metabolism / Endoribonucleases:
genetics / Animals / Protein Serine-Threonine Kinases:
metabolism / Protein Serine-Threonine Kinases: genetics /
Signal Transduction / Brain Neoplasms: pathology / Brain
Neoplasms: metabolism / Myeloid Cells: metabolism / Myeloid
Cells: pathology / Unfolded Protein Response / Tumor
Microenvironment / Tumor Cells, Cultured / Endoplasmic
Reticulum Stress / ER stress (Other) / IRE1 (Other) /
chemokines (Other) / glioblastoma (Other) / inflammation
(Other) / Endoribonucleases (NLM Chemicals) / Protein
Serine-Threonine Kinases (NLM Chemicals) / ERN1 protein,
human (NLM Chemicals)},
cin = {B050},
ddc = {610},
cid = {I:(DE-He78)B050-20160331},
pnm = {312 - Funktionelle und strukturelle Genomforschung
(POF4-312)},
pid = {G:(DE-HGF)POF4-312},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:38153426},
pmc = {pmc:PMC11066906},
doi = {10.1093/neuonc/noad256},
url = {https://inrepo02.dkfz.de/record/290039},
}