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@ARTICLE{Peona:290058,
author = {V. Peona and J. Martelossi and D. Almojil and J. Bocharkina
and I. Brännström and M. Brown and A. Cang and T.
Carrasco-Valenzuela and J. DeVries and M. Doellman and D.
Elsner and P. Espíndola-Hernández and G. F. Montoya and B.
Gaspar and D. Zagorski and P. Hałakuc and B. Ivanovska and
C. Laumer and R. Lehmann and L. L. Boštjančić and R.
Mashoodh and S. Mazzoleni and A. Mouton and M. A. Nilsson
and Y. Pei and G. Potente and P. Provataris$^*$ and J. R.
Pardos-Blas and R. Raut and T. Sbaffi and F. Schwarz and J.
Stapley and L. Stevens and N. Sultana and R. Symonova and M.
S. Tahami and A. Urzì and H. Yang and A. Yusuf and C.
Pecoraro and A. Suh},
title = {{T}eaching transposon classification as a means to crowd
source the curation of repeat annotation - a tardigrade
perspective.},
journal = {Mobile DNA},
volume = {15},
number = {1},
issn = {1759-8753},
address = {London},
publisher = {BioMed Central},
reportid = {DKFZ-2024-00963},
pages = {10},
year = {2024},
note = {DKFZ-ZMBH Alliance},
abstract = {The advancement of sequencing technologies results in the
rapid release of hundreds of new genome assemblies a year
providing unprecedented resources for the study of genome
evolution. Within this context, the significance of in-depth
analyses of repetitive elements, transposable elements (TEs)
in particular, is increasingly recognized in understanding
genome evolution. Despite the plethora of available
bioinformatic tools for identifying and annotating TEs, the
phylogenetic distance of the target species from a curated
and classified database of repetitive element sequences
constrains any automated annotation effort. Moreover, manual
curation of raw repeat libraries is deemed essential due to
the frequent incompleteness of automatically generated
consensus sequences.Here, we present an example of a
crowd-sourcing effort aimed at curating and annotating TE
libraries of two non-model species built around a
collaborative, peer-reviewed teaching process. Manual
curation and classification are time-consuming processes
that offer limited short-term academic rewards and are
typically confined to a few research groups where methods
are taught through hands-on experience. Crowd-sourcing
efforts could therefore offer a significant opportunity to
bridge the gap between learning the methods of curation
effectively and empowering the scientific community with
high-quality, reusable repeat libraries.The collaborative
manual curation of TEs from two tardigrade species, for
which there were no TE libraries available, resulted in the
successful characterization of hundreds of new and diverse
TEs in a reasonable time frame. Our crowd-sourcing setting
can be used as a teaching reference guide for similar
projects: A hidden treasure awaits discovery within
non-model organisms.},
keywords = {Annotation (Other) / Genome assembly (Other) / Library
(Other) / Manual curation (Other) / Non-model organism
(Other) / Transposable elements (Other)},
cin = {W190},
ddc = {570},
cid = {I:(DE-He78)W190-20160331},
pnm = {312 - Funktionelle und strukturelle Genomforschung
(POF4-312)},
pid = {G:(DE-HGF)POF4-312},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:38711146},
doi = {10.1186/s13100-024-00319-8},
url = {https://inrepo02.dkfz.de/record/290058},
}