TY  - JOUR
AU  - Rostami, Sina
AU  - Rounge, Trine B
AU  - Pestarino, Luca
AU  - Lyle, Robert
AU  - Fortner, Renée Turzanski
AU  - Haaland, Øystein Ariansen
AU  - Lie, Rolv T
AU  - Wiklund, Fredrik
AU  - Bjørge, Tone
AU  - Langseth, Hilde
TI  - Differential levels of circulating RNAs prior to endometrial cancer diagnosis.
JO  - International journal of cancer
VL  - 155
IS  - 5
SN  - 0020-7136
CY  - Bognor Regis
PB  - Wiley-Liss
M1  - DKFZ-2024-01007
SP  - 946-956
PY  - 2024
N1  - 2024 Sep 1;155(5):946-956
AB  - Endometrial cancer (EC) is one of the most common female cancers and there is currently no routine screening strategy for early detection. An altered abundance of circulating microRNAs (miRNAs) and other RNA classes have the potential as early cancer biomarkers. We analyzed circulating RNA levels using small RNA sequencing, targeting RNAs in the size range of 17-47 nucleotides, in EC patients with samples collected prior to diagnosis compared to cancer-free controls. The analysis included 316 cases with samples collected 1-11 years prior to EC diagnosis, and 316 matched controls, both from the Janus Serum Bank cohort in Norway. We identified differentially abundant (DA) miRNAs, isomiRs, and small nuclear RNAs between EC cases and controls. The top EC DA miRNAs were miR-155-5p, miR-200b-3p, miR-589-5p, miR-151a-5p, miR-543, miR-485-5p, miR-625-p, and miR-671-3p. miR-200b-3p was previously reported to be among one of the top miRNAs with higher abundance in EC cases. We observed 47, 41, and 32 DA miRNAs for EC interacting with BMI, smoking status, and physical activity, respectively, including two miRNAs (miR-223-3p and miR-29b-3p) interacting with all three factors. The circulating RNAs are altered and show temporal dynamics prior to EC diagnosis. Notably, DA miRNAs for EC had the lowest q-value 4.39-6.66 years before diagnosis. Enrichment analysis of miRNAs showed that signaling pathways Fc epsilon RI, prolactin, toll-like receptor, and VEGF had the strongest associations.
KW  - RNA (Other)
KW  - RNA‐sequencing (Other)
KW  - cell‐free nucleic acids (Other)
KW  - endometrial neoplasms (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:38733362
DO  - DOI:10.1002/ijc.34951
UR  - https://inrepo02.dkfz.de/record/290171
ER  -