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000290486 1001_ $$0P:(DE-He78)106e711f1921fb3ba7defe02f4badd0c$$aHartley, Anna$$b0$$eFirst author$$udkfz
000290486 245__ $$aA Simple Nonviral Method to Generate Human Induced Pluripotent Stem Cells Using SMAR DNA Vectors.
000290486 260__ $$aBasel$$bMDPI$$c2024
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000290486 520__ $$aInduced pluripotent stem cells (iPSCs) are a powerful tool for biomedical research, but their production presents challenges and safety concerns. Yamanaka and Takahashi revolutionised the field by demonstrating that somatic cells could be reprogrammed into pluripotent cells by overexpressing four key factors for a sufficient time. iPSCs are typically generated using viruses or virus-based methods, which have drawbacks such as vector persistence, risk of insertional mutagenesis, and oncogenesis. The application of less harmful nonviral vectors is limited as conventional plasmids cannot deliver the levels or duration of the factors necessary from a single transfection. Hence, plasmids that are most often used for reprogramming employ the potentially oncogenic Epstein-Barr nuclear antigen 1 (EBNA-1) system to ensure adequate levels and persistence of expression. In this study, we explored the use of nonviral SMAR DNA vectors to reprogram human fibroblasts into iPSCs. We show for the first time that iPSCs can be generated using nonviral plasmids without the use of EBNA-1 and that these DNA vectors can provide sufficient expression to induce pluripotency. We describe an optimised reprogramming protocol using these vectors that can produce high-quality iPSCs with comparable pluripotency and cellular function to those generated with viruses or EBNA-1 vectors.
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000290486 650_7 $$2Other$$aS/MAR
000290486 650_7 $$2Other$$aSMAR DNA vector
000290486 650_7 $$2Other$$aiPSC
000290486 650_7 $$2Other$$anonviral
000290486 650_7 $$2Other$$areprogramming
000290486 650_7 $$2Other$$astem cells
000290486 650_7 $$2NLM Chemicals$$aEpstein-Barr Virus Nuclear Antigens
000290486 650_7 $$0O5GA75RST7$$2NLM Chemicals$$aEBV-encoded nuclear antigen 1
000290486 650_2 $$2MeSH$$aInduced Pluripotent Stem Cells: cytology
000290486 650_2 $$2MeSH$$aInduced Pluripotent Stem Cells: metabolism
000290486 650_2 $$2MeSH$$aHumans
000290486 650_2 $$2MeSH$$aGenetic Vectors: genetics
000290486 650_2 $$2MeSH$$aCellular Reprogramming: genetics
000290486 650_2 $$2MeSH$$aFibroblasts: cytology
000290486 650_2 $$2MeSH$$aFibroblasts: metabolism
000290486 650_2 $$2MeSH$$aPlasmids: genetics
000290486 650_2 $$2MeSH$$aEpstein-Barr Virus Nuclear Antigens: genetics
000290486 650_2 $$2MeSH$$aCells, Cultured
000290486 650_2 $$2MeSH$$aTransfection: methods
000290486 7001_ $$0P:(DE-He78)19508ab2203ce13ff769ddaca2ee786e$$aBurger, Luisa$$b1$$udkfz
000290486 7001_ $$0P:(DE-He78)35927260ef999e92f9b5c855daefbd02$$aWincek, Cornelia$$b2$$udkfz
000290486 7001_ $$aDons, Lieke$$b3
000290486 7001_ $$aLi, Tracy$$b4
000290486 7001_ $$0P:(DE-He78)39c2b4b32f9bb19e30cdf4f30020fb63$$aGrewenig, Annabel$$b5$$udkfz
000290486 7001_ $$0P:(DE-He78)97cd7d8ed672718b812c3cf82353c9fd$$aTaşgın, Toros$$b6$$udkfz
000290486 7001_ $$0P:(DE-He78)157a1fcb274700b6427b980f8f4b3e9f$$aUrban, Manuela$$b7
000290486 7001_ $$0P:(DE-HGF)0$$aRoig-Merino, Alicia$$b8
000290486 7001_ $$aGhazvini, Mehrnaz$$b9
000290486 7001_ $$0P:(DE-He78)15dff5647002b4dcfe892b251cd14b62$$aHarbottle, Richard$$b10$$eLast author$$udkfz
000290486 773__ $$0PERI:(DE-600)2527218-4$$a10.3390/genes15050575$$gVol. 15, no. 5, p. 575 -$$n5$$p575$$tGenes$$v15$$x2073-4425$$y2024
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