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@ARTICLE{Hartley:290486,
      author       = {A. Hartley$^*$ and L. Burger$^*$ and C. Wincek$^*$ and L.
                      Dons and T. Li and A. Grewenig$^*$ and T. Taşgın$^*$ and
                      M. Urban$^*$ and A. Roig-Merino$^*$ and M. Ghazvini and R.
                      Harbottle$^*$},
      title        = {{A} {S}imple {N}onviral {M}ethod to {G}enerate {H}uman
                      {I}nduced {P}luripotent {S}tem {C}ells {U}sing {SMAR} {DNA}
                      {V}ectors.},
      journal      = {Genes},
      volume       = {15},
      number       = {5},
      issn         = {2073-4425},
      address      = {Basel},
      publisher    = {MDPI},
      reportid     = {DKFZ-2024-01113},
      pages        = {575},
      year         = {2024},
      note         = {#EA:D420#LA:D420#},
      abstract     = {Induced pluripotent stem cells (iPSCs) are a powerful tool
                      for biomedical research, but their production presents
                      challenges and safety concerns. Yamanaka and Takahashi
                      revolutionised the field by demonstrating that somatic cells
                      could be reprogrammed into pluripotent cells by
                      overexpressing four key factors for a sufficient time. iPSCs
                      are typically generated using viruses or virus-based
                      methods, which have drawbacks such as vector persistence,
                      risk of insertional mutagenesis, and oncogenesis. The
                      application of less harmful nonviral vectors is limited as
                      conventional plasmids cannot deliver the levels or duration
                      of the factors necessary from a single transfection. Hence,
                      plasmids that are most often used for reprogramming employ
                      the potentially oncogenic Epstein-Barr nuclear antigen 1
                      (EBNA-1) system to ensure adequate levels and persistence of
                      expression. In this study, we explored the use of nonviral
                      SMAR DNA vectors to reprogram human fibroblasts into iPSCs.
                      We show for the first time that iPSCs can be generated using
                      nonviral plasmids without the use of EBNA-1 and that these
                      DNA vectors can provide sufficient expression to induce
                      pluripotency. We describe an optimised reprogramming
                      protocol using these vectors that can produce high-quality
                      iPSCs with comparable pluripotency and cellular function to
                      those generated with viruses or EBNA-1 vectors.},
      keywords     = {Induced Pluripotent Stem Cells: cytology / Induced
                      Pluripotent Stem Cells: metabolism / Humans / Genetic
                      Vectors: genetics / Cellular Reprogramming: genetics /
                      Fibroblasts: cytology / Fibroblasts: metabolism / Plasmids:
                      genetics / Epstein-Barr Virus Nuclear Antigens: genetics /
                      Cells, Cultured / Transfection: methods / S/MAR (Other) /
                      SMAR DNA vector (Other) / iPSC (Other) / nonviral (Other) /
                      reprogramming (Other) / stem cells (Other) / Epstein-Barr
                      Virus Nuclear Antigens (NLM Chemicals) / EBV-encoded nuclear
                      antigen 1 (NLM Chemicals)},
      cin          = {D420},
      ddc          = {570},
      cid          = {I:(DE-He78)D420-20160331},
      pnm          = {314 - Immunologie und Krebs (POF4-314)},
      pid          = {G:(DE-HGF)POF4-314},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:38790204},
      pmc          = {pmc:PMC11121542},
      doi          = {10.3390/genes15050575},
      url          = {https://inrepo02.dkfz.de/record/290486},
}