TY  - JOUR
AU  - Cacciotto, Carla
AU  - Dore, Gian Mario
AU  - Cubeddu, Tiziana
AU  - Burrai, Giovanni Pietro
AU  - Anfossi, Antonio Giovanni
AU  - Antuofermo, Elisabetta
AU  - Varoni, Maria Vittoria
AU  - Demontis, Maria Piera
AU  - Zobba, Rosanna
AU  - Pittau, Marco
AU  - Müller, Martin
AU  - Alberti, Alberto
TI  - Ovine papillomavirus type 3 virus-like particle-based tools for diagnosis and detection of infection.
JO  - Vaccine
VL  - 42
IS  - 22
SN  - 0264-410X
CY  - Amsterdam
PB  - Elsevier
M1  - DKFZ-2024-01211
SP  - 126033
PY  - 2024
N1  - 2024 Sep 17;42(22):126033
AB  - The design of prophylactic and diagnostic tools specific to animal papillomaviruses is hampered by the difficulties of viral in vitro manipulation and by the scarce availability of dedicated biotechnological tools. This paper reports the production of Ovine Papillomavirus 3 (OaPV3)-based virus-like particles (OaPV3-VLPs) in the baculovirus system and their use to investigate host humoral immune response through the establishment of an indirect ELISA test., Polyclonal sera and monoclonal antibodies were generated against OaPV3-VLPs, and their isotype and reactivity were determined. Additionally, antibodies allowed OaPV3 detection in ovine squamous cell carcinoma (SCC) samples by immunohistochemistry. Results encourage the standardization of OaPV3-specific prophylactic and serological diagnostic tools, and open new perspectives for the study of host-viral interaction and SCC development.
KW  - Animal papillomavirus (Other)
KW  - Oncovirus (Other)
KW  - Serogical diagnosis (Other)
KW  - Skin cancer (Other)
KW  - Vaccines (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:38839520
DO  - DOI:10.1016/j.vaccine.2024.06.001
UR  - https://inrepo02.dkfz.de/record/290854
ER  -