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@ARTICLE{Cacciotto:290854,
      author       = {C. Cacciotto and G. M. Dore and T. Cubeddu and G. P. Burrai
                      and A. G. Anfossi and E. Antuofermo and M. V. Varoni and M.
                      P. Demontis and R. Zobba and M. Pittau and M. Müller$^*$
                      and A. Alberti},
      title        = {{O}vine papillomavirus type 3 virus-like particle-based
                      tools for diagnosis and detection of infection.},
      journal      = {Vaccine},
      volume       = {42},
      number       = {22},
      issn         = {0264-410X},
      address      = {Amsterdam},
      publisher    = {Elsevier},
      reportid     = {DKFZ-2024-01211},
      pages        = {126033},
      year         = {2024},
      note         = {2024 Sep 17;42(22):126033},
      abstract     = {The design of prophylactic and diagnostic tools specific to
                      animal papillomaviruses is hampered by the difficulties of
                      viral in vitro manipulation and by the scarce availability
                      of dedicated biotechnological tools. This paper reports the
                      production of Ovine Papillomavirus 3 (OaPV3)-based
                      virus-like particles (OaPV3-VLPs) in the baculovirus system
                      and their use to investigate host humoral immune response
                      through the establishment of an indirect ELISA test.,
                      Polyclonal sera and monoclonal antibodies were generated
                      against OaPV3-VLPs, and their isotype and reactivity were
                      determined. Additionally, antibodies allowed OaPV3 detection
                      in ovine squamous cell carcinoma (SCC) samples by
                      immunohistochemistry. Results encourage the standardization
                      of OaPV3-specific prophylactic and serological diagnostic
                      tools, and open new perspectives for the study of host-viral
                      interaction and SCC development.},
      keywords     = {Animal papillomavirus (Other) / Oncovirus (Other) /
                      Serogical diagnosis (Other) / Skin cancer (Other) / Vaccines
                      (Other)},
      cin          = {D335},
      ddc          = {610},
      cid          = {I:(DE-He78)D335-20160331},
      pnm          = {314 - Immunologie und Krebs (POF4-314)},
      pid          = {G:(DE-HGF)POF4-314},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:38839520},
      doi          = {10.1016/j.vaccine.2024.06.001},
      url          = {https://inrepo02.dkfz.de/record/290854},
}