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@ARTICLE{Zuckermann:290898,
author = {M. Zuckermann$^*$ and C. He and J. Andrews and A. Bagchi
and R. Sloan-Henry and B. Bianski and J. Xie and Y. Wang and
N. Twarog and A. Onar-Thomas and K. Ernst$^*$ and L. Yang
and Y. Li and X. Zhu and J. K. Ocasio and K. M. Budd and J.
Dalton and X. Li and D. Chepyala and J. Zhang and K. Xu and
L. Hover and J. T. Roach and K. C. Chan$^*$ and N.
Hofmann$^*$ and P. J. McKinnon and S. Pfister$^*$ and A. A.
Shelat and Z. Rankovic and B. B. Freeman and J. Chiang and
D. Jones$^*$ and C. L. Tinkle and S. J. Baker},
title = {{C}apmatinib is an effective treatment for {MET}-fusion
driven pediatric high-grade glioma and synergizes with
radiotherapy.},
journal = {Molecular cancer},
volume = {23},
number = {1},
issn = {1476-4598},
address = {London},
publisher = {Biomed Central},
reportid = {DKFZ-2024-01237},
pages = {123},
year = {2024},
note = {#EA:B062#EA:B360#LA:B360#},
abstract = {Pediatric-type diffuse high-grade glioma (pHGG) is the most
frequent malignant brain tumor in children and can be
subclassified into multiple entities. Fusion genes
activating the MET receptor tyrosine kinase often occur in
infant-type hemispheric glioma (IHG) but also in other pHGG
and are associated with devastating morbidity and
mortality.To identify new treatment options, we established
and characterized two novel orthotopic mouse models
harboring distinct MET fusions. These included an
immunocompetent, murine allograft model and patient-derived
orthotopic xenografts (PDOX) from a MET-fusion IHG patient
who failed conventional therapy and targeted therapy with
cabozantinib. With these models, we analyzed the efficacy
and pharmacokinetic properties of three MET inhibitors,
capmatinib, crizotinib and cabozantinib, alone or combined
with radiotherapy.Capmatinib showed superior brain
pharmacokinetic properties and greater in vitro and in vivo
efficacy than cabozantinib or crizotinib in both models. The
PDOX models recapitulated the poor efficacy of cabozantinib
experienced by the patient. In contrast, capmatinib extended
survival and induced long-term progression-free survival
when combined with radiotherapy in two complementary mouse
models. Capmatinib treatment increased radiation-induced DNA
double-strand breaks and delayed their repair.We
comprehensively investigated the combination of MET
inhibition and radiotherapy as a novel treatment option for
MET-driven pHGG. Our seminal preclinical data package
includes pharmacokinetic characterization, recapitulation of
clinical outcomes, coinciding results from multiple
complementing in vivo studies, and insights into molecular
mechanism underlying increased efficacy. Taken together, we
demonstrate the groundbreaking efficacy of capmatinib and
radiation as a highly promising concept for future clinical
trials.},
keywords = {Animals / Humans / Glioma: pathology / Glioma: drug therapy
/ Glioma: genetics / Glioma: therapy / Proto-Oncogene
Proteins c-met: antagonists $\&$ inhibitors / Proto-Oncogene
Proteins c-met: genetics / Proto-Oncogene Proteins c-met:
metabolism / Mice / Xenograft Model Antitumor Assays / Brain
Neoplasms: pathology / Brain Neoplasms: drug therapy / Brain
Neoplasms: genetics / Brain Neoplasms: radiotherapy /
Benzamides: pharmacology / Benzamides: therapeutic use /
Cell Line, Tumor / Oncogene Proteins, Fusion: genetics /
Oncogene Proteins, Fusion: metabolism / Female / Protein
Kinase Inhibitors: pharmacology / Protein Kinase Inhibitors:
therapeutic use / Pyridines: pharmacology / Pyridines:
therapeutic use / Crizotinib: pharmacology / Crizotinib:
therapeutic use / Disease Models, Animal / Child / Neoplasm
Grading / Anilides: pharmacology / Imidazoles / Triazines /
Capmatinib (Other) / Combination therapy (Other) / MET
inhibition (Other) / Pediatric-type diffuse high-grade
glioma (Other) / Preclinical trials (Other) /
Radiosensitization (Other) / Proto-Oncogene Proteins c-met
(NLM Chemicals) / capmatinib (NLM Chemicals) / Benzamides
(NLM Chemicals) / Oncogene Proteins, Fusion (NLM Chemicals)
/ MET protein, human (NLM Chemicals) / Protein Kinase
Inhibitors (NLM Chemicals) / Pyridines (NLM Chemicals) /
Crizotinib (NLM Chemicals) / Anilides (NLM Chemicals) /
cabozantinib (NLM Chemicals) / Imidazoles (NLM Chemicals) /
Triazines (NLM Chemicals)},
cin = {B062 / B360 / HD01},
ddc = {570},
cid = {I:(DE-He78)B062-20160331 / I:(DE-He78)B360-20160331 /
I:(DE-He78)HD01-20160331},
pnm = {312 - Funktionelle und strukturelle Genomforschung
(POF4-312)},
pid = {G:(DE-HGF)POF4-312},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:38849845},
pmc = {pmc:PMC11157767},
doi = {10.1186/s12943-024-02027-6},
url = {https://inrepo02.dkfz.de/record/290898},
}