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@ARTICLE{Hasche:291069,
      author       = {D. Hasche$^*$ and M. Hufbauer and I. Braspenning-Wesch$^*$
                      and S. Stephan$^*$ and S. Silling and G. Schmidt$^*$ and S.
                      Krieg$^*$ and A. Kreuter and B. Akgül},
      title        = {{C}ytokeratin-17 expression is commonly observed in
                      keratinocytic skin tumours and controls tissue homeostasis
                      impacting {HPV} protein expression.},
      journal      = {British journal of dermatology},
      volume       = {191},
      number       = {6},
      issn         = {0366-2845},
      address      = {Oxford},
      publisher    = {Oxford University Press},
      reportid     = {DKFZ-2024-01289},
      pages        = {949-963},
      year         = {2024},
      note         = {#EA:F030# / 2024 Nov 18;191(6):949-963},
      abstract     = {The structured expression of several keratins in the skin
                      is associated with differentiation status of the epidermal
                      layers, whereas others are upregulated only during wound
                      healing, in skin disorders and in cancers. One of these
                      stress keratins, K17, is correlated with poor prognosis in
                      various cancer types and its loss has been shown to
                      decelerate tumour growth. K17 expression can also be
                      detected in cutaneous squamous cell carcinomas (SCCs), where
                      UV-irradiation and infection with cutaneous human
                      papillomaviruses (HPVs) are important co-factors. It was
                      previously reported that K17 is upregulated in
                      papillomavirus (PV)-induced benign skin lesions in mice and
                      induces an immunological status that is beneficial for
                      tumour growth.In order to investigate whether K17
                      upregulation is induced by PVs, we analysed K17 levels in
                      skin tumour specimens of different animal models and
                      humans.Various immunofluorescence stainings were performed
                      to identify K17 expression as well as levels of E-Cadherin,
                      vimentin and CD271. Tissues were further analysed by PCRs,
                      qPCRs and ELISA to control for PV activity. K17knockdown
                      cells were generated and effects on viral life cycle were
                      investigated by infection assays, qPCR and Western
                      blotting.We could show that K17 is commonly expressed in
                      skin tumours and that its presence is not directly linked to
                      viral oncoprotein expression. Rather, K17 expression seems
                      to be a marker of epithelial differentiation and its absence
                      in tumour tissue is associated with an
                      epithelial-to-mesenchymal transition. We further showed that
                      the absence of K17 in skin tumours increases markers of
                      cancer stem-like cells and negatively affects viral protein
                      synthesis.Collectively, our data indicate that K17
                      expression is a common feature in skin tumourigenesis. While
                      it is not primarily targeted by PV oncoproteins, our in vivo
                      and in vitro data suggest that it is an important regulator
                      of epithelial differentiation and thus may play a role in
                      controlling viral protein synthesis.},
      cin          = {F030 / W210 / F190},
      ddc          = {610},
      cid          = {I:(DE-He78)F030-20160331 / I:(DE-He78)W210-20160331 /
                      I:(DE-He78)F190-20160331},
      pnm          = {314 - Immunologie und Krebs (POF4-314)},
      pid          = {G:(DE-HGF)POF4-314},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:38878280},
      doi          = {10.1093/bjd/ljae255},
      url          = {https://inrepo02.dkfz.de/record/291069},
}