TY  - JOUR
AU  - Strack, Maren
AU  - Kückelhaus, Jan
AU  - Diebold, Martin
AU  - Wuchter, Patrick
AU  - Huber, Peter E
AU  - Schnell, Oliver
AU  - Sankowski, Roman
AU  - Prinz, Marco
AU  - Grosu, Anca-Ligia
AU  - Heiland, Dieter Henrik
AU  - Nicolay, Nils H
AU  - Rühle, Alexander
TI  - Effects of tumor treating fields (TTFields) on human mesenchymal stromal cells.
JO  - Journal of neuro-oncology
VL  - 169
IS  - 2
SN  - 0167-594X
CY  - Dordrecht [u.a.]
PB  - Springer Science + Business Media B.V
M1  - DKFZ-2024-01323
SP  - 329-340
PY  - 2024
N1  - #LA:E055# / 2024 Sep;169(2):329-340
AB  - Mesenchymal stromal cells (MSCs) within the glioblastoma microenvironment have been shown to promote tumor progression. Tumor Treating Fields (TTFields) are alternating electric fields with low intensity and intermediate frequency that exhibit anti-tumorigenic effects. While the effects of TTFields on glioblastoma cells have been studied previously, nothing is known about the influence of TTFields on MSCs.Single-cell RNA sequencing and immunofluorescence staining were employed to identify glioblastoma-associated MSCs in patient samples. Proliferation and clonogenic survival of human bone marrow-derived MSCs were assessed after TTFields in vitro. MSC' characteristic surface marker expression was determined using flow cytometry, while multi-lineage differentiation potential was examined with immunohistochemistry. Apoptosis was quantified based on caspase-3 and annexin-V/7-AAD levels in flow cytometry, and senescence was assessed with ß-galactosidase staining. MSCs' migratory potential was evaluated with Boyden chamber assays.Single-cell RNA sequencing and immunofluorescence showed the presence of glioblastoma-associated MSCs in patient samples. TTFields significantly reduced proliferation and clonogenic survival of human bone marrow-derived MSCs by up to 60
KW  - Glioma (Other)
KW  - Mesenchymal stem cells (Other)
KW  - Mesenchymal stromal cells (Other)
KW  - Tumor microenvironment (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:38900237
DO  - DOI:10.1007/s11060-024-04740-0
UR  - https://inrepo02.dkfz.de/record/291120
ER  -