%0 Journal Article
%A Makioka, Daiki
%A Inada, Mikio
%A Awano, Masayuki
%A Saito, Ema
%A Shinoda, Takuya
%A Abe, Satoko
%A Yoshimura, Teruki
%A Müller, Martin
%A Sasagawa, Toshiyuki
%A Ito, Etsuro
%T Quantification of HPV16 E7 Oncoproteins in Urine Specimens from Women with Cervical Intraepithelial Neoplasia.
%J Microorganisms
%V 12
%N 6
%@ 2076-2607
%C Basel
%I MDPI
%M DKFZ-2024-01382
%P 1205
%D 2024
%X We present the validity of using an ultrasensitive enzyme-linked immunosorbent assay (ELISA) for quantifying high-risk human papillomavirus (HPV) 16 E7 oncoproteins in urine specimens as a noninvasive method of analyzing the oncogenic activity of HPV. Some reports claim that the oncogenic activity of HPV is a more relevant clinical indicator than the presence of HPV DNA for estimating malignant potential. In the present study, urine containing HPV16 and related types were selected by uniplex E6/E7 polymerase chain reaction and classified according to the pathologic diagnosis of cervical intraepithelial neoplasia (CIN) in cervical biopsy specimens. Our ultrasensitive ELISA was able to detect attomole levels of HPV16 E7 oncoproteins, and it detected HPV16-positive SiHa cells at >500 cells/mL without detecting HPV18-positive cells. Our ELISA results showed E7 oncoproteins in 80
%K E7 oncoprotein (Other)
%K human papillomavirus (Other)
%K noninvasive diagnosis (Other)
%K ultrasensitive ELISA (Other)
%K uniplex E6/E7 PCR (Other)
%K urine (Other)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:38930587
%2 pmc:PMC11205804
%R 10.3390/microorganisms12061205
%U https://inrepo02.dkfz.de/record/291408