TY  - JOUR
AU  - Makioka, Daiki
AU  - Inada, Mikio
AU  - Awano, Masayuki
AU  - Saito, Ema
AU  - Shinoda, Takuya
AU  - Abe, Satoko
AU  - Yoshimura, Teruki
AU  - Müller, Martin
AU  - Sasagawa, Toshiyuki
AU  - Ito, Etsuro
TI  - Quantification of HPV16 E7 Oncoproteins in Urine Specimens from Women with Cervical Intraepithelial Neoplasia.
JO  - Microorganisms
VL  - 12
IS  - 6
SN  - 2076-2607
CY  - Basel
PB  - MDPI
M1  - DKFZ-2024-01382
SP  - 1205
PY  - 2024
AB  - We present the validity of using an ultrasensitive enzyme-linked immunosorbent assay (ELISA) for quantifying high-risk human papillomavirus (HPV) 16 E7 oncoproteins in urine specimens as a noninvasive method of analyzing the oncogenic activity of HPV. Some reports claim that the oncogenic activity of HPV is a more relevant clinical indicator than the presence of HPV DNA for estimating malignant potential. In the present study, urine containing HPV16 and related types were selected by uniplex E6/E7 polymerase chain reaction and classified according to the pathologic diagnosis of cervical intraepithelial neoplasia (CIN) in cervical biopsy specimens. Our ultrasensitive ELISA was able to detect attomole levels of HPV16 E7 oncoproteins, and it detected HPV16-positive SiHa cells at >500 cells/mL without detecting HPV18-positive cells. Our ELISA results showed E7 oncoproteins in 80
KW  - E7 oncoprotein (Other)
KW  - human papillomavirus (Other)
KW  - noninvasive diagnosis (Other)
KW  - ultrasensitive ELISA (Other)
KW  - uniplex E6/E7 PCR (Other)
KW  - urine (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:38930587
C2  - pmc:PMC11205804
DO  - DOI:10.3390/microorganisms12061205
UR  - https://inrepo02.dkfz.de/record/291408
ER  -