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@ARTICLE{Makioka:291408,
      author       = {D. Makioka and M. Inada and M. Awano and E. Saito and T.
                      Shinoda and S. Abe and T. Yoshimura and M. Müller$^*$ and
                      T. Sasagawa and E. Ito},
      title        = {{Q}uantification of {HPV}16 {E}7 {O}ncoproteins in {U}rine
                      {S}pecimens from {W}omen with {C}ervical {I}ntraepithelial
                      {N}eoplasia.},
      journal      = {Microorganisms},
      volume       = {12},
      number       = {6},
      issn         = {2076-2607},
      address      = {Basel},
      publisher    = {MDPI},
      reportid     = {DKFZ-2024-01382},
      pages        = {1205},
      year         = {2024},
      abstract     = {We present the validity of using an ultrasensitive
                      enzyme-linked immunosorbent assay (ELISA) for quantifying
                      high-risk human papillomavirus (HPV) 16 E7 oncoproteins in
                      urine specimens as a noninvasive method of analyzing the
                      oncogenic activity of HPV. Some reports claim that the
                      oncogenic activity of HPV is a more relevant clinical
                      indicator than the presence of HPV DNA for estimating
                      malignant potential. In the present study, urine containing
                      HPV16 and related types were selected by uniplex E6/E7
                      polymerase chain reaction and classified according to the
                      pathologic diagnosis of cervical intraepithelial neoplasia
                      (CIN) in cervical biopsy specimens. Our ultrasensitive ELISA
                      was able to detect attomole levels of HPV16 E7 oncoproteins,
                      and it detected HPV16-positive SiHa cells at >500 cells/mL
                      without detecting HPV18-positive cells. Our ELISA results
                      showed E7 oncoproteins in $80\%$ (4/5) of urine specimens
                      from women with HPV16-positive CIN1, $71\%$ (5/7) of urine
                      specimens from CIN2 patients, and $38\%$ (3/8) of urine
                      specimens from CIN3 patients. Some urine specimens with
                      undetectable E7 oncoproteins were thought to be negative for
                      live HPV 16-positive cells or in an inactivated state of
                      infection. These results provide the basis for assessing
                      oncogenic activity by quantifying E7 oncoproteins in patient
                      urine.},
      keywords     = {E7 oncoprotein (Other) / human papillomavirus (Other) /
                      noninvasive diagnosis (Other) / ultrasensitive ELISA (Other)
                      / uniplex E6/E7 PCR (Other) / urine (Other)},
      cin          = {D335},
      ddc          = {570},
      cid          = {I:(DE-He78)D335-20160331},
      pnm          = {314 - Immunologie und Krebs (POF4-314)},
      pid          = {G:(DE-HGF)POF4-314},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:38930587},
      pmc          = {pmc:PMC11205804},
      doi          = {10.3390/microorganisms12061205},
      url          = {https://inrepo02.dkfz.de/record/291408},
}