TY - JOUR AU - Seckinger, Anja AU - Salwender, Hans AU - Martin, Hans AU - Scheid, Christof AU - Hielscher, Thomas AU - Bertsch, Uta AU - Hummel, Manuela AU - Jauch, Anna AU - Knauf, Wolfgang AU - Emde-Rajaratnam, Martina AU - Beck, Susanne AU - Neben, Kai AU - Dührig, Jan AU - Lindemann, Walter AU - Schmidt-Wolf, Ingo G H AU - Hänel, Mathias AU - Blau, Igor W AU - Weisel, Katja AU - Weinhold, Niels AU - Raab, Marc S AU - Goldschmidt, Hartmut AU - Choon-Quinones, Mimi AU - Hose, Dirk TI - Molecular Long-Term Analysis of the GMMG-HD4 Trial in Multiple Myeloma-Patterns of Association of Chromosomal Aberrations with Response and Proliferation Determining Survival in Selecting Treatments in View of Limited Resources in Low- and Middle-Income Countries. JO - International journal of molecular sciences VL - 25 IS - 12 SN - 1422-0067 CY - Basel PB - Molecular Diversity Preservation International M1 - DKFZ-2024-01385 SP - 6431 PY - 2024 AB - Based on the lack of differences in progression-free and overall survival after a median follow-up of 93 months in our HOVON-65/GMMG-HD4 trial (German part; n = 395) randomizing VAD induction (vincristin/adriamycin/dexamthasone)/tandem-transplantation/thalidomide-maintenance vs. PAD induction (bortezomib/adriamycin/dexamethasone)/tandem transplantation/bortezomib maintenance, we discern how chromosomal aberrations determine long-term prognosis by different patterns of association with proliferation and treatment-dependent response, whether responses achieved by different regimens are equal regarding prognosis, and whether subpopulations of patients could be defined as treatable without upfront 'novel agents' in cases of limited resources, e.g., in low- or middle-income countries. Serum parameters and risk factors were assessed in 395 patients. CD138-purified plasma cells were subjected to fluorescence in situ hybridization (n = 354) and gene expression profiling (n = 204). We found chromosomal aberrations to be associated in four patterns with survival, proliferation, and response: deletion (del) del17p13, del8p21, del13q14, (gain) 1q21+, and translocation t(4;14) (all adverse) associate with higher proliferation. Of these, del17p is associated with an adverse response (pattern 1), and 1q21+, t(4;14), and del13q14 with a treatment-dependent better response (pattern 2). Hyperdiploidy associates with lower proliferation without impacting response or survival (pattern 3). Translocation t(11;14) has no association with survival but a treatment-dependent adverse response (pattern 4). Significantly fewer patients reach a near-complete response or better with 'conventional' (VAD) vs. bortezomib-based treatment after induction or high-dose melphalan. These patients, however, show significantly better median progression-free and overall survival. Molecularly, patients responding to the two regimens differ in gene expression, indicating distinct biological properties of the responding myeloma cells. Patients with normal renal function (89.4 KW - Humans KW - Multiple Myeloma: genetics KW - Multiple Myeloma: drug therapy KW - Multiple Myeloma: mortality KW - Multiple Myeloma: pathology KW - Chromosome Aberrations KW - Female KW - Male KW - Middle Aged KW - Aged KW - Antineoplastic Combined Chemotherapy Protocols: therapeutic use KW - Cell Proliferation: drug effects KW - Prognosis KW - Adult KW - Developing Countries KW - Dexamethasone: therapeutic use KW - Dexamethasone: pharmacology KW - Bortezomib: therapeutic use KW - Bortezomib: pharmacology KW - Thalidomide: therapeutic use KW - LMIC (Other) KW - molecular profiling (Other) KW - multiple myeloma (Other) KW - proliferation (Other) KW - response (Other) KW - survival (Other) KW - Dexamethasone (NLM Chemicals) KW - Bortezomib (NLM Chemicals) KW - Thalidomide (NLM Chemicals) LB - PUB:(DE-HGF)16 C6 - pmid:38928138 C2 - pmc:PMC11204152 DO - DOI:10.3390/ijms25126431 UR - https://inrepo02.dkfz.de/record/291411 ER -