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@ARTICLE{Arnsktter:291648,
author = {F. Arnskötter$^*$ and P. B. G. da Silva$^*$ and M. E.
Schouw$^*$ and C. Lukasch$^*$ and L. Bianchini$^*$ and L.
Sieber$^*$ and J. Garcia-Lopez and S. T. Ahmad and Y. Li and
H. Lin and P. Joshi$^*$ and L. Spänig$^*$ and M. Rados$^*$
and M. Roiuk$^*$ and M. Sepp and M. Zuckermann$^*$ and P. A.
Northcott and A. Patrizi$^*$ and L. Kutscher$^*$},
title = {{L}oss of {E}lp1 in cerebellar granule cell progenitors
models ataxia phenotype of {F}amilial {D}ysautonomia.},
journal = {Neurobiology of disease},
volume = {199},
issn = {0969-9961},
address = {[Amsterdam]},
publisher = {Elsevier},
reportid = {DKFZ-2024-01472},
pages = {106600},
year = {2024},
note = {KFZ-ZMBH Alliance /#EA:B430#LA:B430#},
abstract = {Familial Dysautonomia (FD) is an autosomal recessive
disorder caused by a splice site mutation in the gene ELP1,
which disproportionally affects neurons. While classically
characterized by deficits in sensory and autonomic neurons,
neuronal defects in the central nervous system have also
been described. Although ELP1 expression remains high in the
normal developing and adult cerebellum, its role in
cerebellar development is unknown. To explore the role of
Elp1 in the cerebellum, we knocked out Elp1 in cerebellar
granule cell progenitors (GCPs) and examined the outcome on
animal behavior and cellular composition. We found that
GCP-specific conditional knockout of Elp1 (Elp1cKO) resulted
in ataxia by 8 weeks of age. Cellular characterization
showed that the animals had smaller cerebella with fewer
granule cells. This defect was already apparent as early as
7 days after birth, when Elp1cKO animals also had fewer
mitotic GCPs and shorter Purkinje dendrites. Through
molecular characterization, we found that loss of Elp1 was
associated with an increase in apoptotic cell death and cell
stress pathways in GCPs. Our study demonstrates the
importance of ELP1 in the developing cerebellum, and
suggests that loss of Elp1 in the GC lineage may also play a
role in the progressive ataxia phenotypes of FD patients.},
keywords = {Cerebellum (Other) / Development (Other) / ELP1 (Other) /
Elongator complex (Other) / Familial Dysautonomia (Other) /
Granule cell progenitor (Other) / Neurodevelopmental
disorder (Other) / ataxia (Other)},
cin = {B430 / B140 / B062 / A320},
ddc = {570},
cid = {I:(DE-He78)B430-20160331 / I:(DE-He78)B140-20160331 /
I:(DE-He78)B062-20160331 / I:(DE-He78)A320-20160331},
pnm = {312 - Funktionelle und strukturelle Genomforschung
(POF4-312)},
pid = {G:(DE-HGF)POF4-312},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:38996985},
doi = {10.1016/j.nbd.2024.106600},
url = {https://inrepo02.dkfz.de/record/291648},
}