%0 Journal Article
%A Angelova, Assia Ludmilova
%A Barf, Milena
%A Just, Alexandra
%A Leuchs, Barbara
%A Rommelaere, Jean
%A Ungerechts, Guy
%T H-1 Parvovirus-Induced Oncolysis and Tumor Microenvironment Immune Modulation in a Novel Heterotypic Spheroid Model of Cutaneous T-Cell Lymphoma.
%J Cancers
%V 16
%N 15
%@ 2072-6694
%C Basel
%I MDPI
%M DKFZ-2024-01644
%P 2711
%D 2024
%Z #EA:D490#LA:D490#
%X The rat protoparvovirus H-1 (H-1PV) is an oncolytic virus known for its anticancer properties in laboratory models of various human tumors, including non-Hodgkin lymphomas (NHL) of B-cell origin. However, H-1PV therapeutic potential against hematological malignancies of T-cell origin remains underexplored. The aim of the present study was to conduct a pilot preclinical investigation of H-1PV-mediated oncolytic effects in cutaneous T-cell lymphoma (CTCL), a type of NHL that is urgently calling for innovative therapies. We demonstrated H-1PV productive infection and induction of oncolysis in both classically grown CTCL suspension cultures and in a novel, in vivo-relevant, heterotypic spheroid model, but not in healthy donor controls, including peripheral blood mononuclear cells (PBMCs). H-1PV-mediated oncolysis of CTCL cells was not prevented by Bcl-2 overexpression and was accompanied by increased extracellular ATP release. In CTCL spheroid co-cultures with PBMCs, increased spheroid infiltration with immune cells was detected upon co-culture treatment with the virus. In conclusion, our preclinical data show that H-1PV may hold significant potential as an ingenious viroimmunotherapeutic drug candidate against CTCL.
%K cutaneous T-cell lymphoma (CTCL) (Other)
%K heterotypic CTCL spheroid (Other)
%K oncolytic H-1 parvovirus (Other)
%K virotherapy (Other)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:39123440
%2 pmc:PMC11311363
%R 10.3390/cancers16152711
%U https://inrepo02.dkfz.de/record/292156